Consider an experiment where you digest a DNA molecule with restriction enzyme BclI. You resolve the products of the digest by gel electrophoresis. Then, you cut out each of the resulting two bands from the gel and sequence the DNA. The sequencing results return two DNA segments.
Segment 1
5′ CCGCGGT 3′
3′ GGCGCCACTAG 5′
Segment 2
5′ GATCAGGATCC 3′
3′ TCCTAGG 5′
1. What is the sequence of the BclI recognition site?
2. What type of ends do the products have?

Answers

Answer 1

Answer:

This BclI restriction enzyme recognizes and cut at T / CTAG nucleotide sequences.

Explanation:

5′ CCGCGGT 3′

3′ GGCGCCACTAG 5′

Segment 2

5′ GATCAGGATCC 3′

3′            TCCTAGG 5′

The BclI enzyme generates sticky ends, it means that the enzyme recognises and cuts nucleotide sequences without complementary bases, which also have weak hydrogen bonds. The sticky ends are then generated when an enzyme produces unpaired nucleotide ends. Nonetheless, restriction enzymes generally produce blunt ends, i.e., both strands cut at the same site.


Related Questions

_________16. Which one of the following should NOT be associated with electron transport chain?
A. Absorption of solar energy C. Movement of H+
B. Formation of ATP D. Cytochromes

Answers

The answer to this question will be movement of H+

the brain is located in the ______ cavity?

Answers

its located in the cranial cavity :)

Answer:

cranial Cavity

Explanation:

The brain occupies the cranial cavity, which is lined by the meninges and which contains cerebrovascular fluid to cushion blows.

You have isolated a new mutation in the T4 bacteriophage rII A gene and you map its location within the gene by recombination frequency. You find that this new mutation fails to give wild type recombinants with several different adjacent mutations located in the same gene. What type of mutation is this new one you just isolated

Answers

Answer:

A lethal mutation, thereby the resulting phenotype is not observed .

Explanation:

Lethal mutations are the result of genomic changes that may be lethal in certain conditions. In genetic research, this type of mutation has shown to be very useful in analyzing gene function (i.e., genes whose protein products are key for the survival of the organism in question), and they are perfect gene markers. In bacteriophages, for example, temperature-sensitive mutants of phage T4 that can not grow at temperatures higher 42°C have been identified (Edgar & Lielausis 1964).

Citation:

R. S. Edgar, & I. Lielausis (1964). Temperature-sensitive mutants of bacteriophage T4D: their isolation and genetic characterization. Genetics, 49(4), 649.

How many children and adolescents with histories of maltreatment involving sexual abuse or combined sexual and physical abuse meet criteria for post-traumatic stress disorder (PTSD)?

Answers

Answer:

Exposure to child physical abuse and parents’ domestic violence can subject youth to pervasive traumatic stress and lead to Post-traumatic Stress Disorder (PTSD). The often repeating and ongoing nature of family violence exposure may result in youth exhibiting problems in multiple domains of functioning and meeting criteria for multiple disorders in addition to PTSD. These characteristics as well as unique factors related to 

SCENARIO ABiological anthropologists have discovered a previously unknown fossil species namedAustralopithecus sediba. The species lived about 2 million years ago in Africa and has an interesting mix of traits. For example, its arms are adapted for climbing in trees and its legs are adapted for walking on two legs on the ground. It does not directly resemble any of the living ape species, which suggests that the living ape species (including humans) have each become adapted for their own environmental contexts over time.What is the primary type of biological anthropology addressed in this research?A. Human biologyB. Forensic anthropologyC. PaleoanthropologyD. PrimatologyDoes the research also touch on topics that might be relevant to researchers in disciplines outside anthropology, such as biology, geology, psychology, medicine, sociology, or criminology? Which of the following disciplines would be most relevant?A. PsychologyB. BiologyC. CriminologyD. SociologyHow does this research relate to human evolution? In other words, what can we learn about human evolution from research along these lines?

Answers

Answer:

In scenario A A. Human biology

Which of the following disciplines would be most relevant?

B. Biology

Explanation:

In scenario A Australopithecus sediba arms are adapted for climbing in tree and its legs are adapted for walking on two legs. Australopithecus is related to homo habilis and also homo erectos. They are different human primate.

B. Biology because there are fossil remains dated to about 2 million years ago.

During digestion fat is broken down into

Answers

Answer:

Lipase

Explanation:

Lipase enzymes break down fat into fatty acids and glycerol. Digestion of fat in the small intestine is helped by bile, made in the liver.

Chemical equilibrium results if_____.

Answers

Answer:

what he said ^

Explanation:

i think...

. Why did Jim decide to move his lab to New York? a) he wanted to be near where the trials were occurring b) he wanted to be closer to his family c) the pharmaceutical company demanded it

Answers

Answer:

a) he wanted to be near where the trials were occurring

Explanation:

hope it helps .

Which of the following is made of microcrystalline quartz? Group of answer choices A.dolostone B.gypsum C.chert D.coquina

Answers

c. chert is the answer

2. Mr. Simpson has blood type ABRh+ and his wife type ORh+. Mr. Doodle has type ARh- and his wife type ORh+. The Simpsons and Doodles each had a baby girl on the same day. When they took their children home, their doctors found that the Doodle girl had blood type ARh- and the Simpson girl had type ORh+. The Simpsons sued the hospital for giving them the wrong baby. Could they win the case? Show the most likely genotypes of the parents and of the 2 girls

Answers

ORh+
AbRh+
Because the circumference of the earth and yes it will cause that

Why is axon covered in insulated sheath?

Answers

Answer:  Myelin Sheath. The Myelin Sheath of a neuron consists of fat-containing cells that insulate the axon from electrical activity. This insulation acts to increase the rate of transmission of signals. A gap exists between each myelin sheath cell along the axon.

Explanation:. Myelin can greatly increase the speed of electrical impulses in neurons because it insulates the axon and assembles voltage-gated sodium channel clusters at discrete nodes along its length. Myelin damage causes several neurological diseases, such as multiple sclerosis.

Answer:

Myelin Sheath. The Myelin Sheath of a neuron consists of fat-containing cells that insulate the axon from electrical activity. This insulation acts to increase the rate of transmission of signals. A gap exists between each myelin sheath cell along the axon.

HOPE IT HELPS : D

Explanation:

Why do rocks on the ocean floor form a pattern of magnetized stripes?

Answers

Answer: D trust me on this

Explanation:

the rocks contain iron that points in different directions depending on earths polarity

The polarity of Earth affects the iron in the rocks, which points in various ways, so rocks on the ocean floor form a pattern of magnetized stripes.

How form a pattern of magnetized stripes?

The idea of paleomagnetism, according to which the arrangement of the earth's tectonic plates depends on the iron present in magma, is one of the theories explaining seafloor spreading. The positive and negatively charged ions are represented by the stripes.

The magnetic strips, also known as magnetic anomalies, are identified by white and black strip patterns. They have ions that are positively and negatively charged. As a result, the magma magnetizes with the Earth's magnetic field when it emerges from the ridges.

Therefore, the polarity of Earth affects the iron in the rocks, which points in various ways.

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A cell has two pairs of submetacentric chromosomes, which we will call chromosomes IA, IB, IIA, and IIB (chromosomes IA and I B are homologs, and chromosomes IIA and IIB are homologs). Allele M is located on the long arm of chromosome IA and allele m is located at the same position on chromosome IB. Allele P is located on the short arm of chromosome IA, and allele p is located at the same position on chromosome IB. Allele R is located on chromosome IIA and allele r is located at the same position on chromosome IIB.

A. Draw these chromosomes, identifying alleles M, m, P, p, R, and r as they would appear in metaphase I of meiosis. Assume there is no crossing over.

B. Taking into consideration the random separation of chromosomes in anaphase I, draw the possible types of gametes that might result from this cell’s undergoing meiosis. Assume that there is no crossing over.

Answers

Answer:

A) Allelic position in the chromosomes:

IA  ----------M------------:-----------P----

IB  ----------m------------:-----------p----

IIA -----------------R--------------------:---------------------

IIB -----------------r---------------------:---------------------

B) Types of gametes (assuming there is no crossing over):

MPR; MPr; mpR; mpr.

Explanation:

In submetacentric chromosomes, the centromere is located near to the middle of the chromosomes.

Since these two pairs of homologous chromosomes do not undergo crossover, the resulting gametes after meiosis may have four (4) allele combinations.


The following are examples of characteristics that can be inherited except
(a)intelligence (b) complexion (C) type of blood (d) shape of head (e) sex of the child​

Answers

Answer:

sex of the child

Explanation:

g One can measure ATP production from isolated mitochondria in vitro (in a test tube). Changing the pH of the solution that the mitochondria are in results in a similar change the pH of the intemembrane space. If the pH of the solution that the isolated mitochondria are in is raised and everything else remains constant, what is the effect on ATP synthesis

Answers

Answer:

This will lead to an  decreases  in the ATPs synthesis. This is because low pH , high acidity favours ATP synthesis, because it increases the  proton concentration for electrochemical gradients needed for energy that  ATPase enzymes makes used of   synthesis  of ATPs.

Therefore a rise in the pH(low acidity) lowers protons levels, and therefore reduced electrochemical gradients , with a drop in energy for ATPs synthesis.

Explanation:

which organ of the body doesn't rest?​

Answers

Answer:

Brain

Explanation:

It is always active, and regulates everything going through your body. Even when your asleep, it makes sure that the body is in the right temp, and that all the organs help stay safe.

Answer:

The brain!

Explanation:

The body rests during sleep, not the brain. The brain remains active, gets recharged, and still controls many body functions!

In experiment #2 you are asked to use the scientific method to develop and execute a method of separating a mixture of sand, salt and iron pellets. Imagine you are in a lab and notice that a plate of bacteria grow faster in the presence of sunlight. After that single observation, you come to the conclusion that sunlight causes bacteria to grow fast. What would be the next step in the scientific method for this experiment?

Answers

Answer:

1. separation can be done with help of magnetic bar and filtration.

2. the next step will be testing the hypothesis.

Explanation:

1. Separation of iron pellets from sand,  and salt first

Use a magnet

Due to magnetic force iron pallets will attach with it and separation will occur from sand and salt.

After separation of iron pallets, add water to mixture of sand and salt and heat until salt get dissolved then filter the salt water.

This will help in separating all three components of mixture to separate.

2. Bacteria growth

Hypothesis must be tested-

In this case a hypothesis and conclusion is represented. Conclusion is provided that in presence of sunlight growth of bacteria is faster. So the hypothesis must be verified by scientific experiment to be a valid theory by measuring or monitoring growth in presence of sunlight.

You are doing an experiment and plated E. coliin different medias. Media A has onlyglucose, B has only lactose, and media C has both glucose and lactose.You notice that some of the cultures grown in media C are metabolizinglactose. Which type(s) of mutation(s) could cause this to occur

Answers

Answer:

A. Glucose:

As lactose is absent will follow the glucose metabolism, so the lac repressor will hold tightly with the operator which leads to the prevention of transcription as RNA polymerase can not bind to promoter so no lactose metabolism.

B. lactose:

In the case or wild mutant lactose metabolism will occur as lactose is present, allolactose binds to the lac repressor thus the operator and promoter are free so transcription starts.  The same result will be in all test except in the case of a mutant repressor.

C. glucose and lactose:

In this case, diauxic growth will occur. This growth takes place when two sugars are present in the media. The preferred sugar will be consumed first That is glucose. Then a lag phase will come which will be followed by the second sugar metabolism that is lactose.  the given two cases :

Lactose will be metabolized first, as operator site mutation prevents repressor protein binding and thus will lead to the constitutive synthesis of lac operon in test B.   The same thing will happen, as in this case also, constitutive synthesis of lac operon will happen in test C.

Cultures grown in the presence of both glucose and lactose are metabolizing lactose. In the test (b) and (c) will be responsible due to constitutive synthesis of lac operon will happen.

Describe the type of attitude that would help of attitude that would help to make a good scientist?

Answers

Answer:

My answer to you is that the positive willing to do tests and experiments over and being okay with being wrong if you are.

The transport of water from the roots to the leaves of a green plant against the force of gravity is facilitated by
a. adhesion
b. cohesion
c . surface tension
d . capillary action​

Answers

Nkjkkjhhjkjjjnjjjjjjzz

what type of epithelium has dome shaped on the apical surface? ​

Answers

Answer:

Transitional epithelium has dome shaped cells on the apical surface.

Answer:

Transitional epithelium

Transitional epithelium has dome shaped cells on the apical surface. Pseudostratified epithelium is a type of epithelium that has cells which all touch the basement membrane. Pseudostratified epithelium is only one cell layer thick.

HOPE IT HELPS XD

Explanation:

What happens to the food in small intestine?​

Answers

Answer:

the nutrients in the food is absorbed

Answer:

ExplanatBlood in the villi helps to absorb nutrients and sugar molecules.

Nutrients from food are absorbed.

Fat is broken down into smaller droplets.

Most of the digestion that occurs in the small intestines occurs in the duodenum, which is the first part of the small intestines. This is where fats are broken down into smaller droplets by emulsifiers and lipases. Absorption takes place in the other parts through the villi.ion:

A 45 year old man had coronary artery stents placed 2 days ago. Today, he is in severe distress and reporting crushing chest discomfort.

Answers

Answer:

idk man

Explanation:

Anaplerotic reactions serve to:__________.
a) divert materials out of the citric acid cycle for use in biosynthesis.
b) regulate energy production by bypassing the citric acid cycle.
c) replenish the citric acid cycle if it becomes depleted of intermediates by biosynthetic demands.
d) produce pyruvate, which initiates the cycle when glucose degradation is not occurring.

Answers

Answer: Option C.

replenish the citric acid cycle if it becomes depleted of intermediates by biosynthetic demands.

Explanation:

Anaplerotic reactions that form intermediates for kreb cycle, citric cycle. They form intermediates for metabolic pathways like citric cycle. The citric cycle is important for energy production and biosynthesis. Anaplerotic reactions help to restore or replenish intermediates in citric cycle if there is depletion of intermediates biosynthetic demands.

(a) Describe the possible genotypes of the heart cells of an individual if meiosis in this

individual results in two sperm cells carrying the f allele and two sperm cells carrying the

F allele.

Answers

Answer:

The possible genotype is Ff

Explanation:

The possible genotype of the heart cells of such individual would be Ff.

Meiosis usually results in the formation of daughter cells with haploid number of chromosomes from parent cells with diploid number of chromosomes. The chromosomes in the parent cells are usually halved in the daughter cells.

The cells of the heart will have diploid number of chromosomes because they are vegetative cells while the sperm cells will usually have haploid number of chromosomes because they are sex cells.

Consequently, the heart cells of an individual will contain twice the chromosome number present in the sperm cells of such individual.

Hence, if meiosis results in the formation of sperm cells with F and f genotypes in an individual, it simply means that any diploid cell in that individual will have the Ff genotype.

The possible genotype is Ff

The following information should be considered:

 Meiosis normally arise when the daughter cells are created along with haploid number of chromosomes from parent cells with diploid number of chromosomes. The chromosomes in the parent cells are normally halved in the daughter cells. The heart cells should have diploid number of chromosomes since they are vegetative cells while on the other hand the sperm cells will normally have haploid number of chromosomes. Due to this, the heart cells of an individual will comprise of twice the chromosome number that are present in the sperm cells of such individual.

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Which of these factors will affect the friction on a road?
a) Oil
b) Water
c) Ice
d) all of the above

Answers

Answer:

all of the above

Explanation:

Oil,Water and Ice

all of these will affect the friction on the road.

hope this helps

brainliest appreciated

good luck! have a nice day!

Answer:

D-all of the above

Explanation:

Force of friction is the force which opposes the motion of an object over a surface

The force of friction acts between the object and the surface.

A ball rolling on ground gradually slows down and comes to rest due to force of friction between the ball and the ground.If we stop pedaling a bicycle, it gradually slows down and comes to a stop due to force of friction between the wheel and the road.

Each phospholipid is made up of:
A. Two phosphate groups and one fatty acid chain.
B. A phosphate group and two fatty acid chains.
O
C. A phosphate group and a fatty acid chain.
O
D. Two phosphate groups and two fatty acid chains.

Answers

Answer:

The correct answer is B. A phosphate group and 2 fatty acid chains.

Explanation:

A P3 X

Each phospholipid is made up of a phosphate group and two fatty acid chains. The correct option is B.

What are phospholipids?

Phospholipids are lipids that have two types of edges. It has a hydrophobic tail and a hydrophilic head.  The plasma or cell membrane, according to the fluid mosaic model, comprises substances like proteins and phospholipids.

A phosphate head and a fatty acid (lipid) tail make up the phospholipid layer, also known as the phospholipid layer, which is formed by the phospholipid.

Small molecules like oxygen gas can diffuse through the phospholipid layer, which facilitates the passage of small molecules but not larger ones. Biological membranes are formed by two lipid layers, proteins, and glucans.

Therefore, the correct option is B. A phosphate group and two fatty acid chains.

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24. Explain why water shortages occur frequently in
parts of New York State even though the water
supply is nearly constant. [1]

Answers

Answer:

Water is an essential element of life. Water is life and it has a lot of functions such as for drinking and other domestic purposes. There could however be water shortage in some parts of Nee York states as a result of country as a result of the effects of drought due to low amount of rainfall in the area.

There may also be issues with the various instruments which helps in the transport of water to homes which could also lead to the shortage.

Shortages occur frequently in parts of New York State even though the water supply is nearly constant as there are some issues with the water transport system and also low rainfall in some areas.

What is the way of water transportation?

There can be several methods by which water transportation can be made easy.

Methods of transportation are included under three categories:

Aqueducts: it involves pipelines, canals, tunnels and bridges. Container shipment: it involves tank truck, tank car, and tank ship. Towing: it includes a tugboat is used to pull an iceberg or a large water bag along behind it.

Shortages occur frequently in parts of New York State even though the water supply is nearly constant as there are some issues with the water transport system and also low rainfall in some areas.

Thus, this can be the reason behind water shortage in New York.

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What are the fundamental parts of a typical cell

Answers

A cell consists of these three main parts: the cell membrane, the nucleus, and, between the two, the cytoplasm.

Answer:

the parts include Cell Membrane, Mitochondrion, Lysosome, Rough Endoplasmic Reticulum.    

Explanation:

the cell membrane Is made out of phospholipids and proteins

the Mitochondrion Site of cellular respiration "power house"

the Lysosome S.uicide Sacks that contain digestive enzymes

rough Endoplasmic Reticulum contains Ribosomes, transports proteins

hope this helped!

In rabbits, brown coat (B) is dominant to white coat (b). A rabbit breeder wants to know the genotype of his best looking male brown rabbit using a test cross. He has brown females and white females available to conduct his breeding experiments.

Required:
a. What should be the coat color of the female he must use in this test cross?
b. If the test cross produces seven (7) brown rabbits and one (1) white rabbit, what is the genotype of the brown male rabbit?

Answers

Answer:

a. white

b. Bb

Explanation:

A test cross usually involve crossing an individual whose zygosity is unknown with a homozygous recessive version of individual of the same species.

Brown coat (B) is dominant over white coat (b). Hence, there is no way to physically distinguish between a BB rabbit and a Bb  rabbit because both of them will be brown. A bb rabbit however will be white in appearance.

A test cross would therefore involve crossing the brown male rabbit with bb (white) rabbit.

Assuming that the genotype of the brown rabbit is BB, all the progeny will be brown as shown by the cross below.

         BB   x   bb

    Bb   Bb   Bb   Bb

In order word, assuming the genotype is Bb, some of the offspring will appear brown while some will appear white.

      Bb  x   bb

Bb   Bb   bb   bb

Hence, the only way the test cross would produce white offspring is if the genotype of the brown rabbit is heterozygous, Bb.

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can anyone say me the answer please The shape of the path of a planet or asteroid around the sun is an._____ 7. Bud and his friends stopped at a local fast food restaurant after the football game Friday night. Bud ate a one-fourth pound cheeseburger and a large order of fries. The cheese30 grams of total fat. If the recommended daily allowance of total fat for a 2,000 calorie diet is 64 grams, what percent of the daily allowance did Bud have when he ate the cheeseburger School speech for class 7 what was the decision in plessy v. ferguson? Kier Company issued $600,000 in bonds on January 1, Year 1. The bonds were issued at face value and carried a 5-year term to maturity. The bonds have a 6.00% stated rate of interest and interest is payable in cash on December 31 each year. Based on this information alone, what are the amounts of interest expense and cash flows from operating activities, respectively, that will be reported in the financial statements for the year ending December 31, Year 1 On October 1, Natalie King organized Real Solutions, a new consulting firm. On October 31, the company's records show the following items and amounts.Cash $2,000 Cash dividends $3,360Accounts receivable 13,000 Consulting fees earned 15,000Office supplies 4,250 Rent expense 2,550Land 36,000 Salaries expense 6,000Office equipment 28,000 Telephone expense 660Accounts payable 7,500 Miscellaneous expenses 680Common stock 74,000Also assume the following:a. The owners initial investment consists of $37,720 cash and $45,940 in land in exchange for its common stock. b. The companys $17,710 equipment purchase is paid in cash. c. The accounts payable balance of $8,230 consists of the $2,990 office supplies purchase and $5,240 in employee salaries yet to be paid. d. The companys rent, telephone, and miscellaneous expenses are paid in cash. e. No cash has been collected on the $13,800 consulting fees earned.Required:Using the above information to prepare an October 31 statement of cash flows for Real Solutions. WILL GIVE BRAINIEST!!Scientists use models of earthquakes to (5 points)change their locationstop them from occurringunderstand the processincrease the waves formed in the process Analyze the set below and answer the question that follows. (KUM) n (KUL) Which Venn diagram has shading that represents the given set? What type of mixture is fog?O A. A homogenous mixture of a solid in a gasB. A heterogeneous mixture of a liquid in a gasC. A homogenous mixture of a liquid in a gasD. A heterogeneous mixture of a solid in a gas What is the equation of the line that is parallel to the line 5x + 2y = 12 and passes through the point (-2, 4)?Oy=-5/2x-1O y=-5/2x+5Oy=2/5x-1Oy=2/5x+5 580 is divided between Sushil, Fiona & Pip so that Sushil gets twice as much as Fiona, and Fiona gets three times as much as Pip. How much does Fiona get? Use the Pythagorean Theorem to find the length of the hypotenuse in the triangle shown below.6025 Why can a liquid take the shape of the bottom of its container?A: The particles of a liquid can vibrate within their fixed locationsB: The particles of a liquid can break free from one another and spread outC: The particles of a liquid can flow around one another to a new location Read the following poem by Emily Dickinson.Water, is taught by thirst.Land-by the Oceans passed.Transport-by throe-Peace-by its battles told-Love, by Memorial Mold-Birds, by the Snow.Which words in this poem rhyme?"throe" and "Snow""Love" and "Mold""Peace" and "told" Dacia asks Katarina why it is important to learn a new coordinate system, because they have been using the Cartesian coordinate system and it seems to Dacia that it works fine. Which of Katarina's replies to Dacia are correct? write a thank u letter to ur uncle for gifting a criclet bat A circle has a radius of \blue{3}3start color #6495ed, 3, end color #6495ed. An arc in this circle has a central angle of 20^\circ20 20, degrees. Which statement best describes the impact of 20th century conflicts such as World War I and World War II? HELPP ?Air at a temperature of 27 C and 1 atm pressure in a 4 liter cylinder of a diesel engine There. By pushing the piston, the volume of air shrinks 16 times and the pressure increases 40 times. a) How many moles of air are in the cylinder. b) What is the final temperature of the air?