In the ELISA procedure, first a protein sample is immobilized to a plastic well. Then, the antibody to the protein of interest is added, incubated, and the excess is washed out. Next, the detection antibody (which binds to the primary antibody) is added, incubated, and the excess is washed out. The last step is to add the detection dye (which binds to the detection antibody) to the wells and allow it to incubate. What do you predict the result would look like if you added the detection antibody first, then the antibody to the protein of interest

Answers

Answer 1

Answer:

In this ELISA procedure, a sample of protein is immobilized in the plastic well and then antibody for this protein added and incubated, and cleared out. In the given setting detection, the antibody is conjugated with the protein of interest and if the substrate molecule added to the reaction it will produce a colored product by reacting with the enzyme.

No reaction will take place in the well in case of not adding secondary antibody or detection antibody to the reaction and no colored product will be received.


Related Questions

Your skin is an example of a nonspecific defense mechanism. Why is it called a nonspecific defense mechanism?

A. becuase it protects against any invader regardless of type
B. because it has to be 'alerted' that a foreign invader has been spotted
C. because it alerts the immune system when a foreign invader is spotted
D. because it protects against a specific type of pathogen

Answers

Answer:

A is the answer

Explanation:

This is what skin does, it blocks outside material from getting into the body regardless of what it is.

SOLVE THIS QUESTION IS SUPER IMPORTANT

Answers

Answer: The genes that can give you red-green color blindness are passed down on the X chromosome. Since it's passed down on the X chromosome, red-green color blindness is more common in men. This is because: Males have only 1 X chromosome, from their mother.

Explanation:

the cytoplasmic extensions that, together with the cell body, provide the main receptive surfaces for neurons are the:

a: axons
b: soma
c: dendrites
d: synapses

Answers

I’m pretty sure it’s dendrites
:)

This sunbird has built a nest.
How does this behavior affect the survival of the sunbird population?
O A. It keeps offspring warm as they grow and develop
B. It communicates to the offspring how to reproduce.
O C. It teaches offspring how to build their own nests.
D. It helps attract a mate and produce offspring.

Answers

Answer:

your answer is  d i think

Explanation:

Answer:

It's A

Explanation:

I got it right, this one is the most logical one since the purpose of a nest is to keep the offspring warm as they grow and develop.

Hope this helps!!

A bacteria structure involved in motility.

Answers

Answer:

flagella

Explanation:

Thin long thread like structure that help in locomotion in bacteria.

After a carnivore feeds on an organism, the rest of the dead body is __________.
Select one:

eaten by parasites

used by producers

invaded by viruses

consumed by decomposers

Answers

The correct answer would be D

After a carnivore feeds on an organism, the rest of the dead body is: D. consumed by decomposers.

What is a carnivore?

A carnivore is also referred to as a carnivorous animal and it can be defined as a type of animal whose food and energy requirements are primarily derived from animal products, especially by hunting and scavenging.

This ultimately implies that, a carnivore is an animal that only feeds on other animals such as deers, cows, zebras, rather than grasses or plants.

In conclusion, decomposers are a group of microorganism such as fungi and bacteria, that consumes the rest of the body of a dead or decaying organism after a carnivore has fed on it.

Read more on carnivore here: brainly.com/question/13571764

URGENT!!!!!! PLZ HELP!!!!!!

An organism and its taxonomic key are shown below.

Taxonomic Key

Step Characteristic Organism

1 8 legs --- Go to step 2.

More than 8 legs --- Go to step 3.

2 One body region --- Go to step 4.

Two body regions --- Go to step 5.

3 One pair of legs on each body segment ---- Centipede

Two pairs of legs on each body segment ------ Millipede

4 Less than 1 millimeter long --- Mite

More than 1 millimeter long --- Tick

5 Claw-like pinchers --- Go to step 6.

No claw-like pinchers --- Spider

6 Long tail with a stinger --- Scorpion

No tail or stinger --- Pseudoscorpion

Which organism is shown in the image?

A. Millipede
B. Spider
C. Scorpion
D. Tick

Answers

It’s literally a spider

1. Does the insect have wings? a. Yes………………………………………………………………………… go to step 2 b. No…………………………………………………………………………. Order Hemiptera 2. Does the insect have parallel wings? (hint: Parallel wings don’t stick out to the side) a. Yes……………………………………………………………………………… go to step 3 b. No………………………………………………………………………………. go to step 4 3. Does the insect have a parallel line down the back that divides the wings? a. Yes…………………………………………………………………………… Order Coleoptera b. No …………………………………………………………………………… Order Orthoptera 4. Does the insect have 4 total wings? a. Yes………………………………………………………………………………… go to step 5 b. No………………………………………………………………………………… Order Diptera 5. Does the insect have long antennae? a. Yes………………………………………………………………………………go to step 6 b. No……………………………………………………………………………… Order Odonata 6. Does the insect have a small body with large fan –shaped wings? a. Yes…………………………………………………………………………… Order Lepidoptera b. No………………………………………………………………………………Order Hymenoptera

Answers

Complete question: You will find the image in the attached files

Based on the dichotomous key below, what order does this insect belong to?

1. Does the insect have wings?  

a. Yes………………………………………………………………………… go to step 2

b. No…………………………………………………………………………. Order Hemiptera

2. Does the insect have parallel wings? (hint: Parallel wings don’t stick out to the side)

a. Yes……………………………………………………………………………… go to step 3

b. No………………………………………………………………………………. go to step 4

3. Does the insect have a parallel line down the back that divides the wings?

a. Yes…………………………………………………………………………… Order Coleoptera

b. No …………………………………………………………………………… Order Orthoptera

4. Does the insect have 4 total wings?

a. Yes………………………………………………………………………………… go to step 5

b. No………………………………………………………………………………… Order Diptera

5. Does the insect have long antennae?

a. Yes………………………………………………………………………………go to step 6

b. No……………………………………………………………………………… Order Odonata

6. Does the insect have a small body with large fan –shaped wings?

a. Yes…………………………………………………………………………… Order Lepidoptera

b. No………………………………………………………………………………Order Hymenoptera

Answer:  

ORDER ODONATA

Explanation:

It is simple to read a dichotomous key. You only need to understand the technical terminology and to detailed observe your specimen. The key will lead you to the answer.

1. Does the insect have wings?

a. Yes………………………………………………………………………… go to step 2

b. No…………………………………………………………………………. Order Hemiptera

We only need to answer this question by looking at the image. In our example, yes, the insect has wings. So we need to go to question number two. Note: In the hypothetical case that the insect would not have wings, it would belong to the Hemiptera order.

2. Does the insect have parallel wings? (hint: Parallel wings don’t stick out to the side)

a. Yes……………………………………………………………………………… go to step 3

b. No………………………………………………………………………………. go to step 4

Ok, here it tells you that parallel wings do not stick out to the side. But in our example, wings do stick out, so the answer should be NO. This species does not have parallel wings. As it is a NO, we go to question number 4.

Note: In the hypothetical case that the insect would have parallel wings, we should read number 3.

4. Does the insect have 4 total wings?

a. Yes………………………………………………………………………………… go to step 5

b. No………………………………………………………………………………… Order Diptera

This one is easy. How many wings our specimen has? Four. Then it is a YES, and we go to question number 5. Note: In the hypothetical case that the insect would not have 4 wings, it would belong to the Diptera group.

5. Does the insect have long antennae?

a. Yes………………………………………………………………………………go to step 6

b. No……………………………………………………………………………… Order Odonata

Our insect has very short antennae. They are not long at all. So, the answer is NO, the insect does not have long antennae. This answer leads us directly to the order of our individual. ORDER ODONATA. Note: In the hypothetical case that the insect would have long antennae, we should read question number 6. And so on until we would reach the order.  

ORDER ODONATA:

Dragonflies belong to this group. These insects characterize as having big eyes, and the separation between them depends on the species. They have an elongated abdomen, sometimes even longer than the wings. Their bodies must be robust to support the massive musculature that propels the wings during flight. The wings are long, big, and wide.

Usually, the males are much more colored than the females are.

1.
Which has the greatest impact on the temperature of the Earth's atmosphere?
chemical runoff
sulfur dioxide
carbon dioxide
Ozone

Answers

The answer is carbon dioxide.

A cell that contains 10% NaCl is sitting in a solution of 30% NaCl. Due to osmosis which direction will water move?

Into and out of the cell at equal rates

Out of the cell

There will be no water movement

Into the cell

Answers

Answer:

I think out of the cell!

Hope it helps..

pls help me with answers due today

Answers

it's hard to read, can you crop out the rest so it's just the worksheet

If the amino acids glycine, alanine and valine were combined in one molecule, what would the molecule be called?

Answers

Answer:

A tripeptide

Explanation:

Alanine (Ala), Glycine (Gly) and Valine (Val) are amino acids, i.e., organic molecules that contain at least an amino (–NH2) and one carboxyl (–COOH) functional group. The amino acids held together by peptide bonds, which are formed by a carboxyl group of one amino acid and an amino group of another one, in order to form a peptide. Thus, a tripeptide is a peptide composed of three amino acids joined by two internal (and sometimes three) peptide bonds. In the case above described, the tripeptide formed by the binding of Glycine, Alanine, and Valine can be abbreviated as Gly-Ala-Val (i.e., in this order).

which of the following is a function of the spleen?​

Answers

Answer:

Clearance of microorganisms and particulate antigens from the blood stream.

Synthesis of immunoglobulin G (IgG), properdin (an essential component of the alternate pathway of complement activation), and tuftsin (an immunostimulatory tetrapeptide)

Removal of abnormal red blood cells (RBCs)

Explanation:

what is not a trend evident to plant evolution

Answers

Answer:

b. spores of two types to spores of one type

Explanation:

Spores of two types to spores of one type is not the trend to plant evolution because first the spores of one type is present in living organisms but with the passage of time evolution occurs that will leads to formation of  spores of two types happen in living organisms. There are types of spores present in plants such as microspores, which produces male gametophytes, and megaspores, which produce female gametophytes while on the other hand, spores of one type is present in plants having asexual reproduction.

PLEASE HELP TAKING TEST NOW!!!!!!!!!!!!!! Many animals influence and contribute to ecosystem services. As pollinators, how do bees ultimately contribute to direct ecosystem services? (1 point)
O Bees pollinate plant species that contribute to the carbon cycle.
O Bees pollinate plant species that humans admire in nature.
Bees pollinate plant species that produce oxygen that humans need.
O Bees pollinate plant species that produce food.

Answers

Answer:

D

Explanation:

Many animals influence and contribute to ecosystem services. As pollinators, how do bees ultimately contribute to direct ecosystem services? (1 point)

O Bees pollinate plant species that contribute to the carbon cycle.

O Bees pollinate plant species that humans admire in nature.

Bees pollinate plant species that produce oxygen that humans need.

O Bees pollinate plant species that produce food.

the measurement of the matter in the universe is called

Answers

Answer: mass

Explanation:

In all the laboratories the benches are placed at right angles to windows. Why

Answers

Answer:

in case of emergency

Explanation:

Question 10 of 10
Which of these is a cost of desalination technology?
O A. New supply of fresh drinking water
B. Thousands of suitable locations worldwide
O C. Inexhaustible supply of ocean water
D. Enormous energy requirements

Answers

Answer:

maybe it could be C?

Explanation:

i dont know why?

U
A plant breeder is breeding flowers. She researches the plants she is using 2 points
and discovers that pink is dominant to orange for this particular flower.
She crosses two plants: a heterozygous pink plant and an orange plant. *
75% pink and 25% orange
50% pink and 50% orange
100% orange
100% pink

Answers

Answer:

the answer should be ( 50% pink and 50% orange )

for better understanding u can make a cross yourself

if you want me to show that then I can put an attachment.

Group the following into the appropriate range of measurement.
a. atomic
b. ultramicroscopic
c. microscopic
d. macroscopic
Match each of the options above to the items below.
1. roundworm, algae
2. protozoan, rickettsia
3. herpesvirus, DNA
4. hydrogen atom, glucose molecule

Answers

Answer:

Hydrogen atom & glucose molecule --- atomic.

Herpesvirus, DNA --- ultramicroscopic.

Protozoan, Rickettsia - microscopic.

Roundworm and algae --- macroscopic

Explanation:

1) Hydrogen atom & glucose molecule --- atomic.

The atomic size level, which interacts with atoms as well as small molecules, is much lower than the ultramicroscopic range.

2) Herpesvirus, DNA --- ultramicroscopic.

Ultramicroscopic particles are very tiny structures that cannot be seen using a conventional optical microscope, necessitating the use of an electron microscope. Viruses and DNA falls into this group since they are much smaller than tiny species like bacteria.

3) Protozoan, Rickettsia - microscopic.

Microscopic species are much smaller than macroscopic organisms because they cannot be observed by the naked human eye and must be viewed through a microscope. The size range for microscopic particles is [tex]( 10^3 \ \ to \ \ 10^{10})[/tex], which contains protozoans and Rickettsia (a gram-negative, non-motile bacteria genus).

4) Roundworm and algae --- macroscopic

Naked eyes can see macroscopic organisms; for example, several types of circular worms and algae can be seen by them.

PSYCH Having a family history involving anxiety disorder, experiencing or witnessing a traumatic event, or having a history of abuse are all __________ of anxiety disorders.
A. common symptoms
B. risk factors
C. causes
D. thinking styles

Answers

The answer is B. RISK FACTORS

Having a family history involving anxiety disorder, experiencing or witnessing a traumatic event, or having a record of abuse are all risk factors of anxiety disorders.

Which type of anxiety disorder could be connected to traumatic past events?

Post-traumatic stress disorder is a cognitive health disorder that's begun by a scary event either sharing it or witnessing it.

Signs may include flashbacks, demons and severe anxiety, as well as unruly thoughts about the affair.

Thus, option "B"  risk factors is correct.

To learn more about anxiety disorder click here:

https://brainly.com/question/1049467

#SPJ2

What carries nutrients and oxygen from the mother to the fetus.
A. Umbilical cord
B. Fallopian Tubes

Answers

A. umbilical cord is the answer

Answer:

Umbilical Cord

Explanation:

The unbilical cord uses the blood of the mother to carry oxygen to the blood of the fetus through the placenta.

Consider the following scenario:

A drought hits the habitat of a semi-aquatic bird population. All ponds dry up, and fish populations decline. There are two groups of birds in the population that differ in leg length and diet. Long-legged birds eat fish, while short-legged birds eat insects. The drought has little effect on insect populations.


What is the main selective pressure in this scenario?

(1 point)

drought

leg length

insect population

fish population





There are two varieties in a population of squirrels: short tails and long tails. Long-tailed squirrels are better at jumping and balancing in trees. Scientists observe that over the span of 100 years, the proportion of the population with long tails increases from 10% to 60%. Which statement is most likely? (1 point)

The proportion of long-tailed squirrels may have increased due to increased food availability.

The proportion of short-tailed squirrels may have decreased due to decreased food availability.

The proportion of short-tailed squirrels may have decreased due to an increase in trees.

The proportion of long-tailed squirrels may have increased due to a decrease in trees.

Answers

1. What is the main selective pressure in this scenario?

fish population.

2. According to the question, option "A" is correct The proportion of long-tailed squirrels may have increased due to increased food availability.

What is selective pressure?

In evolutionary theory, the effect on survival of a species of the sum of all elements, physical and behavioural, inherent and environmental especially as an inherited characteristic may marginally affect survival under the influence of these elements.

Thus, the option "D" and "A" is correct.

To learn more about selective pressure click here:

https://brainly.com/question/7091258

One thousand offspring were typed. This cross produced 430 red-eye, wild-type wing flies, 65 purple-eye wild-type wing flies, 60 red-eye, vestigial wing flies, and 445 purple-eye, vestigial wing flies. What is the distance between the loci for purple eyes and vestigial wings in centiMorgans (map units)

Answers

Complete question:

In Drosophila, red eyes is wild-type and purple eyes are mutant. Full-size wings are wild-type and vestigial wings are mutant. Flies with wild-type pr pr vg vg genotypes were crossed with double mutant flies with a pr-pr- vg-vg- genotypes. The F1 flies had pr pr- vg vg- genotypes. The F1 flies with heterozygous genotypes were crossed with double mutant flies with a pr-pr- vg-vg- genotypes One thousand offspring were typed. This cross produced 430 red-eye, wild-type wing flies, 65 purple-eye wild-type wing flies, 60 red-eye, vestigial wing flies, and 445 purple-eye, vestigial wing flies. What is the distance between the loci for purple eyes and vestigial wings in centiMorgans (map units)

Answer:

Distance between genes → 12.5 MU = 12.5 cM.

Explanation:

You will find the complete explanation in the attached files due to technical problems.

Select all that apply.
Tumors can result from:

1) Mutations in tumor suppressor genes
2) Mutations in proto-oncogenes
3) Too many cells in GO
4) Too little cell death
5) Too much cell division

Answers

4, 5 I think I’m not too sure

Why is a shell considered to be biotic?

Answers

A shell is considered as biotic (living) because it is a hard, rigid covering of many animals such as snail, sepia, pila, turtles, sea urchins. A shell is protective outer layer of these soft bodied animals.

it is considered to be biotic because it is known to be a protector for the living thing. it covers the living things skin to keep itself protected. if you need this answer more in depth it’s on quizlet!

During meiosis the cell replicates its DNA once, but divides twice. So each cell only ends up
with half the DNA.
MITOSIS
MEIOSIS
88
V3
1. Mitosis makes
daughter cells.
1. Meiosis makes
daughter cells.
chromosomes
2. Each daughter cell has
inside it.
2. Each daughter cell has chromosomes
in it, which is half of the parent cell.
3. Are the daughter cells identical to each
other?
3. Are all the daughter cells identical to each
other?

Answers

Explanation:

in mitosis the daughter cells are genetically identical to each other but in meiosis the daughter cells aren't identical, it has half of the chromosome of its parent cell unlike mitosis who have exactly same number of chromosome as it parent

Hello who’s good at biology guys I need someone to help me with the test

Answers

I Dont Even Know If Am Good In Biology Or Not

But Some Chums Might Give You A Hand

Question 10 of 10
Why do fungi need to live in moist areas?
A. Their large surface areas make them dry out easily.
B. They can only reproduce sexually.
C. They are unable to photosynthesize.
D. Their filaments make it difficult to absorb nutrients.

Answers

Answer:

A. Their large surface areas make them dry out easily.

Explanation:

(A.P.E.X)

14. Kidneys are filters. As blood passes through the kidney, waste in the form of urine is
left behind. Tubes take the urine from the kidney to a storage bag called the bladder.
What can you conclude about blood leaving the kidney?
A. It moves to the bladder.
B. It has no water left in it.
C. It is ready to be used by the body.
D. It needs to have urine added to it

Answers

Answer:

D. It needs to have urine in it

Explanation:

They are located just below the rib cage, one on each side of your spine. Healthy kidneys filter about a half cup of blood every minute, removing wastes and extra water to make urine. The urine flows from the kidneys to the bladder through two thin tubes of muscle called ureters, one on each side of your bladder. The fluid that is filtered out from the blood then travels down a tiny tube-like structure called a tubule. The tubule adjusts the level of salts, water, and wastes that will leave the body in the urine. Filtered blood leaves the kidney through the renal vein and flows back to the heart.

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it is about the Mayan people PLEASE help Word Bank :CondensationEvaporation Precipitation Sublimation Transportation Using the works from the Word Bank, explain how the water cycle works. You must using complete sentences and using all of the words in the word bank. Please write you answer in 5 or more sentences using words used from the word bank. besties helpAs a good citizen should, Mary votes in every election that she can. She votes for her representative to the legislature, but is not allowed to vote for the leader of the government. What type of government system does Mary vote in? A. communism B. presidential democracy C. parliamentary democracy D. autocracy Sally had some marbles. After buying 12 more marbles, she had 25 marbles. How many marbles did Sally have before she bought more? If a heavy box represents a molecule which of the following represents passive transport in a cell PLSSSS HELP GIVING 5 POINTS PLUS BRAINLIEST Which statement best completes the diagram? ? Countries in Oceania maintain good international relations. O A. Oceania's economies rely on trade. B. Oceania's territory is often invaded. O C. Oceania's populations are enormous. O D. Oceania's militaries are very strong. Which equation below had a solution x =5.5 ?O A) -1 + x = 6.5OB) -6x = -33OC) -3x = 16.5OD)-2 + x = -7.5 How is ADPconverted to ATP? Jake wants to buy two pairs of a particular style of sunglasses. The sunglasses are sold at two different stores. Sunglass House sells the sunglasses go 20% off the $40 retail price. The Eyewear Shoppe sells the sunglasses for $60 but is having a Buy 1 Pair, Get 1 Pair Free promotion. Which store has the better buy? Someone help me pls :( Anyone please, thanks please help asap, i will mark as brainliest. please try to find the answer! asap Simplify: 6a - 2(5a - 6) Discuss the importance of the change in CO with exercise? Marcus changed jobs after college. His old salary was $48000 per year. Now his new salary is 37% more per year. What is his new salary? How many gallons of water does it take to produce the following:a. Cheeseburgerb. Pound of butterc. A pair of jeans Poease help! Thank you in evolutionary terms, what is fitness? _______ are considered to be fluids. SolidsLiquids onlyGases onlyLiquids and gases Clara has 3 bottles of paint. She buys 5 more bottles of paint. If she uses 1/4 bottle of paint to paint a picture, how many similar paintings can she paint with all the paint?ANSWER:She has 3+5=8 bottles of paint. 1/4 bottle paints a picture.8 divided by 1/4 = 32 paintings possible with all the paint