The generation of a knock-out organism generally requires all the following EXCEPT ________. The generation of a knock-out organism generally requires all the following EXCEPT ________. knowledge of the sequence product embryonic stem cells knowledge of the target sequence a targeting vector

Answers

Answer 1

Answer:

knowledge of the sequence product

Explanation:

A gene knockout is a technique used in molecular genetics to deactivate target genes in an organism in order to study their functions by reverse genetics (i.e., gene loss). Knockouts are generated by different methods including, for example, homologous recombination or site-specific nucleases (zinc-fingers, TALENS, CRISPR/Cas9). These techniques require to know a priori the sequence of each gene to be knocked out in order to target desired mutations. In the last years, the CRISPR/Cas9 tool has gained attention to knockout genes of interest because it is a genome editing system that can be easily used for deletion or insertion of bases.


Related Questions

Which of these zones of the ocean is deepest?
A. Abyssopelagic
B. Epipelagic
C.Hadalpelagic
D. Bathypelagic

Answers

hadalpelagic is the zone of the ocean is deepest.

Which of the following is a frameshift mutation from the original DNA of CGCATTGGA?


a. CGCATTGGA

b. CGGCATTGGA

c. CGCATTGGT

d. GGCATTGGA

Answers

Answer: b.

Explanation:

Cgc,att,gga - original

cg(g)c,att,gga. Frame shift

cgc,att,gg(t) snp

(g)gc,att,gga snp

what is the main function of nervous tissue? a: to transport materials and defend the body, b. send signals to control the body, c. contract the casue movement in the body, d. cover the body to protect other cells

Answers

Answer: to contract to cause movement in the body

Explanation:

AP*X

The main function of nervous tissue C. contract causes movement in the body.

What is the main function of the nervous system and how is it structured?

The nervous system is a complex collection of nerves and specialized cells known as neurons that transmit signals between different parts of the body. It is essentially the body's electrical wiring. Structurally, the nervous system has two components: the central nervous system and the peripheral nervous system.

Conclusion: The nervous system is a highly integrated system. The nervous system has three overlapping functions based on sensory input, integration, and motor output. At a more integrative level, the primary function of the nervous system is to control and communicate information throughout the body.

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HELPPP ITS DUE IN A HOUR!!!

Answers

Answer:

below

Explanation:

Radiation Transfer which is the fission of atoms, and conduction, which is the solidarity of atoms

You are given a protein with the following amino acid sequence:
methionine (nonpolar)-glycine (nonpolar)-serine (polar)-alanine (nonpolar)-lysine (polar)
If an amino acid substitution occurred in this polypeptide chain, predict which option would have the most
significant impact on the structure and function of the protein.
a. Serine is replaced by lysine (polar)
b. Glycine is replaced by alanine (nonpolar)
c. Alanine is replaced by leucine (nonpolar)
d. Lysine is replaced by tryptophan (nonpolar)
?

Answers

Answer:

 d. Lysine is replaced by tryptophan (nonpolar)

Explanation:

Amino acid substitutions can have varying affects on the protein function, depending on the specific substitution and the location of the substitution.

If the substitution occurs in an important region, for example an enzyme active site, then the function of the protein could be destroyed.

Additionally, if the substitution replaces the amino acid with a highly different amino acid with different properties (eg. polarity), it could alter the structure and function of the protein.

Replacing lysine (polar) with a tryptophan (non-polar) changes the properties of the amino acid at that location, whereas the other substitutions do not.

Which phrase describes an ecosystem?
A) a population of fish in a small lake
B) the wood of a rotting log
C) an entire desert, including abiotic and biotic factors
D) the community of organisms within a desert

Answers

Answer:

an entire desert, including abiotic and biotic factors

Explanation:

A ecosystem is a place where living things live.

Hope this helps <3

C

Process of elimination

what is the major biomolecule of cell membrane?​

Answers

Answer:

hope it helps...

Explanation:

The principal components of the plasma membrane are lipids (phospholipids and cholesterol), proteins, and carbohydrate groups that are attached to some of the lipids and proteins. A phospholipid is a lipid made of glycerol, two fatty acid tails, and a phosphate-linked head group.

IF THIS HELPED PLEASE COMMENT..

One factor that determines the amount of oxygen transferred from the lungs to the blood is
the total functional surface area of the respiratory membrane.
O True
O False

Answers

Answer:

true

Explanation:

What type of cells are produced during meiosis?

Answers

Answer:

gamete cells :)

Explanation:

hope this helps

Haploid cells are produced during meiosis.

Identical haploid daughter cells are produced from diploid parent cell.This process is required to produce egg and sperm cells for s exual reproduction.

What is meiosis?Meiosis is a type of cell division that reduces the number of chromosomes in the parent cell by half and produces four gamete cells. This process is required to produce egg and sperm cells for             s exual reproduction.Cell division occurs twice during meiosis, one starting cell can produce four gametes.In each round of division, cells go through four stages: prophase, metaphase, anaphase, and telophase.

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what risk does liquefaction pose during an earthquake?

Answers

Answer:

It can turn loose soil into a liquid during an earthquake. supports of buildings, bridges, pipelines, and roads, causing them to sink into the ground, collapse or dissolve.

Explanation:

It can transform into soil

One goal of scientists is to assign every organism a universally accepted name according to the system known as

Answers

Answer:

Binomial Nomenclature

Explanation:

Karl Line is the creator of the binomial nomenclature, which leads to uniqueness in naming plants and categorizing plants into classes, orders, genders, and species.

Binomial nomenclature in biology denotes the official method of naming species. As the word itself suggests, the scientific name is formed by a combination of two expressions: the name of a genus and a species.

Which molecule is a high energy output of photosynthesis

Answers

I think it’s ATP I searched it

One difference between weather and climate is that _____. climate is localized, and weather occurs over large areas. weather is localized, and climate occurs over large areas. weather is the day-to-day conditions, and climate is the conditions expected over relatively long periods of time. climate is the day-to-day conditions, and weather is the conditions expected over relatively long periods of time.

Answers

Answer:

Weather is the day-to-day conditions, and climate is the conditions expected over relatively long periods of time.

Explanation:

The difference between weather and climate is how long the period of time they refer to is.

Weather is what conditions of the atmosphere are over a short period of time, or day-to-day conditions. It's what we see on the weather forecast every day - whether it's going to be sunny or rainy today and tomorrow and similar.

Climate is what conditions of the atmosphere are over longer periods of time, usually 30 years. In fact, it's an average pattern of weather for a particular region. A description of a region's climate will include the precipitation, temperature, humidity, sunshine, wind velocity, phenomena such as fog, frost, and hail storms, and similar conditions.

How can we conserve energy and instead of wasting energy?

Answers

Answer:

Adjust your day-to-day behaviors.

Replace your light bulbs.

Use smart power strips.

Install a programmable or smart thermostat.

Purchase energy efficient appliances.

A study was conducted to understand the factors controlling the rate at
which molecules or ions travel across cell membranes. An artificial
membrane was created that was composed of a phospholipid bilayer only.
The speed at which various substances crossed this membrane was
measured. Some substances can pass through an actual cell membrane
much faster than they passed through the artificial membrane in this study
Which of the following statements best explains this finding?"

a) Actual cell membranes have a much thicker phospholipid bilayer than the artificial
membrane does

b) Actual cell membranes have a variety of proteins embedded in the membrane that are
absent in the artificial membrane

c) Hydrophobic substances spend more time between the two layers of phospholipid in
the artificial membrane than they do between the layers in an actual membrane

d) Hydrophilic substand spend more time attached to the polar region of the
phospholipids in the artificial membrane than they do attached to the polar region of
the phospholipid in an actual membrane

Answers

Answer:

b) Actual cell membranes have a variety of proteins embedded in the membrane that are

absent in the artificial membrane

Explanation:

Which choice below is an example of humans modifying the physical environment?

Farmers clearing land to plant

Transportation improvements

Commercial development

Industrial development

Answers

Industrial development!

Digestion and breathing are _____. controlled by ganglia and plexus reflexive actions controlled by the spinal cord voluntary actions controlled by the central nervous sytem

Answers

Answer:

The correct answer is - reflexive actions controlled by the spinal cord.

Explanation:

Digestion and breathing are examples of involuntary action which is controlled by the autonomic nervous system of the CNS. however, the part of the nervous system that controls is the spinal cord and hind brain by the spinal reflex actions.

These are the action they do not require any thought and work without any thought that leaves the brain for other actions and coordinations. specific but the same stimuli causes the response in the same way.

Are all nerve cells are connected to the brain?

Answers

Answer:

yes

Explanation:

Answer: Is the brain made up of nerve cells?

The brain is what it is because of the structural and functional properties of interconnected neurons. The mammalian brain contains between 100 million and 100 billion neurons, depending on the species. Each mammalian neuron consists of a cell body, dendrites, and an axon.

Explanation: Have a blessed day!

SOMEONE PLZ HELP!!!!!!!!!

Answers

Answer:

the central vacuole.

Explanation:

the vacuole is responsible for discarding waste within the cell.

Answer:

central vacoule because it is just like a vacum :)

Explanation:

I've been stuck on this for a while, any help?

Answers

Explanation:

wheres the question? haha๑๑

Please help it’s urgent!!!!

Answers

Answer:

The first answer

Explanation: “add Energy from the form of heat to get a change in the state of matter”

Which is the overall purpose of the excretory system? extracting energy from food removing wastes from the body moving nutrients through the body gathering information

Answers

Answer:

removing wastes from the body

Explanation:

Excretory system is one of the organ systems in our body. The excretory system encompasses all the organs in the body associated with the function of REMOVING WASTES FROM THE BODY, which represents the primary function of the excretory system.

These organs in the excretory system which include; kidney, skin, lungs etc. all functions to remove one waste or the other. For example, the kidney removes URINE, the skin removes SWEAT, the lungs remove C02.

Answer:

removing wastes from the body

Explanation:

edge 2021

Put the steps in the proper order to explain the flow of information from the DNA sequence to a functional protein.

1. The codon sequence in mRNA determines the amino acid sequence in a polypeptide.
2. Each codon in mRNA codes for a specific amino acid.
3. The sequence of amino acids in a polypeptide determines how the protein will fold and possibly bind with other polypeptides to form a mature protein.
4. The final structure of a protein determines its function.
5. Each three nucleotides in the gene template DNA codes for a three nucleotide codon in mRNA.

Answers

Answer:

5. Each three nucleotides in the gene template DNA codes for a three nucleotide codon in mRNA.

2. Each codon in mRNA codes for a specific amino acid.

1. The codon sequence in mRNA determines the amino acid sequence in a polypeptide.

3. The sequence of amino acids in a polypeptide determines how the protein will fold and possibly bind with other polypeptides to form a mature protein.

4. The final structure of a protein determines its function.

Explanation:

The process of gene expression follows the central dogma of biology I.e. Gene (DNA) --> mRNA ---> Protein. The process of gene forming mRNA is called transcription while the process of mRNA forming protein is called translation.

Based on this, the order of the flow of information from the DNA sequence to a functional protein is:

- Each three nucleotides in the gene template DNA codes for a three nucleotide codon in mRNA (transcription).

- Each codon in mRNA codes for a specific amino acid.

- The codon sequence in mRNA determines the amino acid sequence in a polypeptide (translation).

- The sequence of amino acids in a polypeptide determines how the protein will fold and possibly bind with other polypeptides to form a mature protein.

- The final structure of a proteiin determines its function.

The order is 'three nucleotides in DNA codes for a codon', 'each codon codes an amino acid', 'codon sequence determine amino acid sequence', 'amino acids determine protein folding' and 'final protein structure determines its function'.

During gene transcription, a fragment of DNA referred to as 'gene' is used as a template to create an mRNA, which is then used to synthesize a polypeptide.

The triplets of nucleotides or 'codons' in the mRNA sequence determine the amino acid sequence in the nascent polypeptide chain.

The synthesized protein is then folded in the endoplasmic reticulum (ER) and thus adopts its three-dimensional (3D) conformation.

Protein folding is achieved by specific proteins called chaperones that help to correctly fold the protein, which adopts a mature conformational active state.

In conclusion, the order is 'three nucleotides in DNA codes for a codon', 'each codon codes an amino acid', 'codon sequence determine amino acid sequence', 'amino acids determine protein folding' and 'final protein structure determines its function' (order 52134).

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All of the phases of mitosis may be observed on one whitefish blastula or onion root tip slide. a) true b) false

Answers

Answer:

its true

Explanation:

how is a bacterium different from ours

Answers

Answer:

Viruses are tinier than bacteria. In fact, the largest virus is smaller than the smallest bacterium. All viruses have is a protein coat and a core of genetic material, either RNA or DNA. Unlike bacteria, viruses can't survive without a host.

Explanation:

Answer:

Viruses are tinier than bacteria. In fact, the largest virus is smaller than the smallest bacterium. All viruses have is a protein coat and a core of genetic material, either RNA or DNA. Unlike bacteria, viruses can't survive without a host.

Explanation:

Which division has a single preganglionic neurons with many axon collaterals that forms 20 or more synapses with postganglionic neurons?

a. sympathetic motor division
b. parasympathetic motor division
c. sympathetic sensory division
d. parasympathetic sensory division

Answers

Answer:

i believe its c

Explanation:

A single sympathetic preganglionic fiber has many axon collaterals and may synapse with 20 or more postganglionic neurons.

The postganglionic axons typically terminate in several visceral effectors and therefore the effects of sympathetic stimulation are more widespread than the effects of parasympathetic stimulation.

NEED HELP RLLY BAD!!!!!
Which of the following is not an example of a lipid?
a. triglyceride
b. steroid
c. enzyme
d. phospholipid

Answers

Answer:

c. enzyme

Explanation:  lipids are oily or wax-like   organic molecules found in living  things . They  are what make up fat  and oils

The answer is C. Enzyme

Why is VRBA (Violet red bile agar) only pasteurized rather than sterilized prior to use?

Answers

Answer:

Explanation:

This is because Violet and red bike is only a selective medium that detect and analyse lactose coliform microorganisms that cause fermentation and this is present in diary products like milk. This is milk use for microbiological analysis of milk because of it ability to detect lactose which is a milk sugar. There fore it cannot be use for sterilization.

. Oleander is a very hardy plant that can survive in a wide
variety of climates. It is also known to have very distinct
hydrotropic behavior. Explain how this may be problematic if
the oleander is planted near a home or business.

Answers

Answer: you have to tend to it for 1 hour ever day and make sure there are no weed taking the water.

Explanation:

What values or parameters can you adjust in this computer model?

Answers

Hi, you've asked an incomplete question. However, I provided some information about what a computer model does.

Explanation:

Remember, a computer model in simple terms is a representation of a real-life process or system using a computer. In other words, the computer model is a virtual representation of reality.

For example, weather forecasters often make use of computer models in other to predict changes in weather by taking note of certain values or parameters.

Answer:

You can adjust the type of fluid used, the amount of the fluid, and the amount of water.

Explanation:

PLATO

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