The primers you used in lab amplified 145 base pairs that were adjacent, but not included in the repeat. If you used a different primer that only amplified 130 base pairs adjacent to a 14 base pair repeat, and you had 4 repeats in your product, what was the size of your final PCR product?

186

214

158

200

172

Answers

Answer 1

Answer:

186

Explanation:

The Polymerase Chain Reaction (PCR) is a technique used in molecular biology to amplify small segments of DNA. This technique uses a pair of oligonucleotide sequences called 'primers' which bind to opposite strands of the heat-denatured DNA template in order to amplify this target DNA sequence. In this case, the DNA template is composed of a single unit of 130 base pairs (bp) and four repetitive units of 14 bp, so the final PCR product will have 186 bp >>> 130  bp + (14 bp x 4) = 130 + 56 = 186.


Related Questions

1 point
7.A tree has organs that carry out specific functions needed for survival.
What function do the tree's leaves carry out? *

A)act as reproductive structures

B)capture sunlight to make food

C)take in dissolved minerals

D)anchor the tree underneath the soil

Answers

Answer:

C sorry if im wrong

Explanation:

.

Consider the following chemical reaction: Hb + O2 → HbO2 When this reaction is going from right to left, what process is occurring?

O a. oxygen unloading

O b. cellular respiration

O c. pulmonary ventilation

O d.oxygen loading

Answers

Answer: A, Oxygen Unloading

Identical twins, who have inherited a gene associated with obesity, are adopted by different families. One is growing up in a very health-conscious family, while the other is growing up in a family that eats a lot of fast food and never exercises. Which statement describes their likely weights upon reaching adulthood?

A.
They will both be overweight because they both have the gene associated with obesity.

B.
They will both be of average weight because their genes will mutate as they grow and develop.

C.
The weight of the twins is difficult to predict because both genes and environmental factors affect weight.

D.
One twin will be overweight and the other will be of average weight, because they were raised in different environments.

Answers

Answer:

c

Explanation:

this statement is true and no matter what the genes say environmental parts play a huge role

22. What is the advantage of cells being so small?

A. Small cells contain a greater quantity of enzymes than large cells.

B. Small cells do not require energy and get everything they need from osmosis.

C. The cell has a smaller surface area to volume ratio which means it can move nutrients into
the cell and waste out more efficiently.

D. The cell then has a larger surface area to volume ratio which means it can move nutrients
into the cell and waste out more efficiently.

PLEASE EXPLAIN WHY

Answers

Answer:

B. Small cells do not require energy and get everything they need from osmosis.

Explanation:

Small cells contain a greater quantity of enzymes than large cells. The cell has a smaller surface area to volume ratio which means it can move nutrients into the cell and waste out more efficiently.

lol helplpppppppppppppp

Answers

Answer:

Metamorphic for first

Igneous for secomd

Sedimentary for third

Explanation:

Pls vote me as brainlest!

First one is igneous rocks

Second one is metamorphic rocks

The third one is sedimentary rocks

True or false--
All the modern species of bison have evolved overtime just as all living organisms evolve over time.

Answers

Answer:

The working assumption for many years was that, like North American bison, European bison evolved from steppe bison. ... Their results suggest that the European bison is the result of inter-breeding, over 120,000 years ago, between steppe bison and aurochs; the wild ancestors of modern domestic cattle.

Our study suggests that the first bison arrived in North America 195 - 135 thousand years ago over a land bridge between Asia and Alaska. They quickly spread and evolved (changed) into different forms to adapt to their new environments.

*insert JIMIN'S ROCK BISON pic here*

Explanation:

I.d.k what answer ur expecting from this sentence army.

so I just gave more info regarding this..lol.

A man has type AB+ blood who has a who is heterozygous for the Rh factor. A woman has type O+ blood
and is heterozygous for the Rh factor.
a. What are the chances the offspring is type A blood? ______
b. What are the chances the offspring is type B blood? ______
c. What are the chances the offspring is type AB blood? ______
d. What are the chances the offspring is type O blood? ______
e. What are the changes the offspring is +? ______

Answers

Answer:

A, 1/2

B, 1/2

C, 0

D, 0

E, 1/1

Explanation:

With O+ and AB+ the only possible combinations, via a punnett square, are A+ and B+.

What are the characteristics of iodine ?

Answers

Iodine is a non-metallic, dark-gray/purple-black, lustrous, solid element.

A forest has many trees and bushes that produce nuts. This is a major food sources for many species, including a particular bird. Birds of this species have a variety of beak shapes ranging from long and pointy to short and hard beaks. The birds with short and hard beaks are able to eat the nuts better than the birds with long and pointy beaks. According to this scenario and the principles of natural selection what will happen to this bird population over time. Group of answer choices

Answers

Answer:

There are four principles at work in evolution—variation, inheritance, selection and time. These are considered the components of the evolutionary mechanism of natural selection. After a while the birds will get old and their beaks will get weaker, eventually they will die, when there is a shortage of food they will start harming each other in order for it, that’s selection.

Explanation:

What are 2 kingdoms of bacteria

Answers

Answer:

Eubacteria and Archaea

Explanation:

ht tps://www.encyclopedia.com/science/encyclopedias-almanacs-transcripts-and-maps/bacterial-kingdoms

delete the space between https to read the article! Hope it helps! Maybe a brainliest?

Please help me please

Answers

Answer:

index fossils are fossils used to identify different geological periods of time. they are important for scientists to understand the time frame of which they're collecting strata from. criteria that make thes fossils are being easy to recognize, large in number and size


An original DNA sequence reads ATTCATGAC, and the mutated sequence
reads ATTCGTGAC. What type of mutation is this?

Answers

The type of mutation is Substitution.
It’s substitution because the letter or part of the sequence was replaced/changed with a different letter, (A was replaced with G in the sequence).
Hope this helps and leave a comment if you need any clarification :)

The shark still has identical skeleton to previous sharks. What other way can you prove evolution occurred if fossil evidence does not show any?

Answers

There is biogeography comparative and anatomy and comparative embryology and molecular biology

5. After a massive clean-up, why will contamination continue for decades after?

Answers

Answer:

This is Because they can't be 100% in location of PCBs since animals could spread and reproduce among different places with these toxins within their systems.

Explanation:

Which phyla have well developed nervous system

Answers

Explanation:

Cnidarians

.............



Complete the complementary strand of DNA, using the same symbols for phosphates (circles), sugars (pentagons), and bases. Mark the 5' and 3' direction of the strands,

Drag the appropriate labels to their respective targets. Terms can be used once, more than once, or not at all

Answers

About the question:

You will find the completed strand in the attached files

Answer and Explanation:

First, we need to recognize each strand, the original one and the complementary one. We can recognize the original strand because bases are already given. So,

The original strand is the one at the left,The complementary strand is the one at the right.

Now we need to recognize and pair the bases. Names are written with their letters.

Nitrogenated bases that form nucleic acids correspond to purines and pyrimidines. Adenine and guanine derive from purines, while Thymine and Citocine derive from Pyrimidines.  

In the DNA molecule, Adenine (Purine) forms pairs with Timine (Pyrimidine), while Guanine (Purin) pairs with Cytosine. Two hydrogen bonds join the A-T pair, and three hydrogen bonds join the G-C.

Once we drag the base labels to their targets, we need to recognize each of the strain extremes.

One of the extremes has a phosphate group, which is named 5´. The other extreme has a hydroxyl OH named 3´. Both of them are readen in the 5´to 3´ direction. The polymerase enzyme reads the original strand in 5´ to 3´ mode, and the molecule lecture goes from 3´to 5´.

So, by looking at the labels, we will recognize the phosphate extreme and the hydroxyl one. We will place in the superior extreme of the original strain the phosphate group 5´ and in the other extreme the OH 3´. These positions will give us the direction reflected by the arrow. The complementary strand will have the same extreme labels but in opposite places. So first we will place the OH 3´ label in the superior extreme, and the phosphate 5´label in the inferior extreme, defining the direction reflected by the arrow.

(c) Based on the data, describe the time period during which Chlorella approximates exponential growth. Based on
the data and assuming logistic growth, describe the time period during which the Chlorella population has reached
the carrying capacity of the culture. Based the data, calculate the growth rate per day for the 5-day period with
the greatest growth rate.

Answers

Populations that experience a logistic growth model depend on the density and available resources. A) 5 - 10 days. B) 25 - 30 days. C) 0.27 x 10⁶cells/mL/day

What is the logistic growth model?

In a logistic growth model, the population growth depends on density. Natality and mortality depend on the population size, meaning that there is no independence between population growth and population density.

When a population grows in a limited space, density rises gradually and eventually affects the multiplication rate. The population per capita growth rate decreases as population size increases.

The population reaches a maximum point delimited by available resources, such as food or space. This point is known as the carrying capacity, K.

K is a constant that equals population size at the equilibrium point, in which the natality and the mortality rate get qual to each other.

Assuming that the population size is N, when

N<K, the population can still grow. N approximates to K, the population's growth speed decreases. N=K the population reaches equilibrium, N>K, the population must decrease in size because there are not enough resources to maintain that size.  

The sigmoid curve represents the logistic growth model.

In the example,

Between 0 and 5 days the population remains relatively stableBetween 5 and 10 days Chlorella approximates exponential growth.Between 10 and 20 days Chlorella experiences exponential growth. Between 20 and 25 days the population is near the K and decreases its growth rate.From 25 to 30 days, the population reached the equilibrium point, and got to stabilize.  

Time period during which Chlorella,

          A) approximates exponential growth ⇒ Between 5 and 10 days

Between 10 and 20 days its already exponential growth.

         B) has reached the carrying capacity of the culture ⇒ 25 to 30 days

Finally,

        C) Growth rate per day for the 5-day period with the greatest growth rate.

First we need to figure out during which period Chlorella experiences the  greatest growth rate. To do it, we just need to make the following substraction,

Growth rate per period = final concentration - initial concentration

Period 0-5 ⇒ 0.08 - 0.01 = 0.07Period 5-10 ⇒ 0.55 - 0.08 = 0.47Period 10-15 ⇒ 1.9 - 0.55 = 1.35Period 15-20 ⇒ 2.8 - 1.9 = 0.9Period 20-25 ⇒ 3.2 - 2.8 = 0.4Period 25-30 ⇒ 3.2 - 3.2 = 0  

Now we know that during the period from 10 to 15 days, the population experienced its greatest growth rate. But this rate is acchieved in 5 days. Now we need to find out the growth rate per day. So all we need to do, is to divide the result of the substraction per 5 days,

1.35 x 10⁶ / 5 days = 0.27 x 10⁶ cells/mL/day

The answer is 0.27 x 10⁶ cells/mL/day

You can learn more about logistic growth model at

https://brainly.com/question/16354435

https://brainly.com/question/15631218

Evidence for Evolution:Quesuull The hip bone in whales, the leg bone in snakes, and the appendix in humans are examples of:​

Answers

Answer:

The correct answer is - vestigial structures or organs.

Explanation:

Vestigial organs are structures or the organs are body parts that had once important function but now have no apparent function. These are body parts that look like these are residual parts from a past ancestor.

The hip bone in whales, the leg bone in snakes, and the appendix in humans are thought to have once important functions but now have no apparent function and are vestigial organs.

hi please help i’ll give brainliest

Answers

Answer:

C the moom rotates around itself slower than eath rotates around itself

Explanation:

name two tissues which are thickened with lignin​

Answers

Selerenchyma and xylem vessels?

Answer: parenchyma (living thin-walled tissue) and collenchyma

Explanation:

Explain how the release of energy (cooling) affects the speed of the particles in a substance

Answers

Answer: When a substance is heated, it gains thermal energy. Therefore, its particles move faster and its temperature rises. When a substance is cooled, it loses thermal energy, which causes its particles to move more slowly and its temperature to drop.

Explanation:

Which of the following is an example of a private effort to help the environment? *
A. An individual donates money for a park.
B. A business recycles its waste.
C. A nonprofit group buys land for preservation.
D. all of the above

Answers

Answer:

The correct answer is - D. all of the above.

Explanation:

Any effort from a private sector made for the preserve or help for the betterment of the environment and ecology. Establishing and making a park will help in increasing plant diversity which increases the air quality of the ecosystem and atmosphere.

An individual donates money for the park. Recycling the waste of a private business is also hep in maintaining the environment. Preserving the land also helps the environment.

?????????? helppppppppp

Answers

Answer:

Nuclear Energy from uranium is non-renewable

Hydrogen from water is renewable

Wind energy from air is renewable

Solar energy from the Sun is renewable

Fossil fuel from coal is non-renewable

Explanation:

Energy resources refers to those materials or items from which energy can be derived. Energy resources are generally classified into renewable and non renewable energy resources.

Renewable energy resources are those types of resources which can be renewed or replenished as they are being used up. Renewable energy is often referred to as clean energy and comes from natural sources or processes. Examples of these renewable energy resources are energy from wind, water and sunlight.

Non-renewable energy resources are those types of resources which are not easily renewed or replenished as they are being used up. Examples include energy from fossil fuels such as petroleum and natural gas, coal, nuclear energy from radioactive materials, etc.

From the given table:

Nuclear Energy from uranium is non-renewable

Hydrogen from water is renewable

Wind energy from air is renewable

Solar energy from the Sun is renewable

Fossil fuel from coal is non-renewable

Propose an explanation for why DNA is transcribed
into RNA rather than directly into a proteins

Answers

DNA has to be transcribed into RNA because not only can dna not leave the nuclear for translation at the ribosomes
Also for translation to occur the codons on the mRNA match up with the codons on the tRNA which is connected to the amino acids forming the chain

PLEASE HELP ASAP I WILL MARK AS BRAINLIEST!
Give an example of a chemical reaction that is caused when reactants cool.

Answers

Answer:   iron and oxygen combining to make rust. vinegar and baking soda combining to make sodium acetate, carbon dioxide and water.

Explanation:

Which is a shorter process - (circle one) selective breeding or natural selection?
Explain your answer.

Answers

Answer:

Selective breeding.

Explanation:

Both are fairly long processes, however, the measurement for this is not just the duration to obtain the target, but also the exact target obtained. In natural selection, the duration may be faster than selective breeding in obtaining a change, but the determined change may not be the one that is sought out for. It may have a small chance, but it is not guaranteed. In selective breeding, on the other hand, the destination is determined, and all steps work towards the objective. So in a sense, selective breeding has the greater percentage chance of being the shorter process.

~

What option would be a good adaptation for an animal in a dense RAINFOREST? *
A. heavy body
B. ability to sprint
C. ability to blend with multiple colors and patterns

Answers

Answer:

c because they need to live instead of dying. bc from other bigger animals they can see it and eventually die so.

Question 4

2 pts

The number of chemical industries along Florida's rivers is increasing. What is the most

likely consequence of this increased industrialization?

O a decrease in the amount of water pollution

O an increase in the amount of water available for recreational use

an increase in the destruction of natural food webs

O a decrease int he amount of water needed by industry

Answers

Answer:

The correct answer would be - an increase in the destruction of natural food webs.

Explanation:

Increasing chemical industries near the river cause harmful effects to the environment by various means, including air pollution, dumping untreated chemical or industrial waste, and wastewater in the river. This will lead to a lack of potable water, contamination of the food chain, destruction of biodiversity, and many more.

The destruction of biodiversity can be caused by the increasing the unbridled growth of phytoplankton, eutrophication process that will consume more oxygen and cause algal bloom and decrease the oxygen in the water and destroys the organism and natural food web.

Which is not a property of an acid?
A)
tastes sour
(B) has a high pH
(C)reacts with metals
D contains the element hydrogen

Answers

It is B. Don't open that link. They've posted same link on multiple questions so I assume it's not safe.

Solar system included all of the following except what?

Answers

Answer: Stars?

Explanation: I think it is stars because there is only one star in our solar system. I don't know for sure if it is, but I think it is. Don't be too sure of my answer.

Other Questions
How can you call something "alive"? All living things share certain characteristics.Part A: List the 9 characteristics of life. (1 point)Part B: Choose 3 of the characteristics of life above. Give an example of each that describes how that characteristic contributes to the organism's survival. (3 points) Read the passage.April Fools' Days Around the WorldApril Fools' Day is celebrated around the world, although it varies somewhat from country to country. In the United States, for example, it's a day to play practical jokes. In France, where the holiday is known as Poisson d'Avril, or April Fish, people secretly tape paper fish on their friends' backs. A common April Fools' prank in Portugal is to throw flour on someone; in Scotland, the most popular trick involves sending someone on a useless errand. Denmark celebrates April Fools' Day on April 1, but some people there celebrate again in May. Maj-kat, on May 1, is yet another day for jokes and pranks in the spring.What is the main idea of the passage?Though there are variations is how differences in how different countries observe April Fool's Day, the celebrations all involve playing pranks.On Poisson April, France's version of what Americans call April Fool's Day, pranksters tape paper fish to people's backs. What is the difference between Independent and Dependent events? A. In Dependent - each event relies on the event before it.B. They are the same - independent just means more events are occurring.C. I. independent - each event relies on the event before it.D. They are the same - dependent just means more events are occurring. How many moles of Cs are contained in 3 moles of Cs3PO4? Hey guy pls help me with dis due today pls help no links plsI did number one pls explain answer write an equivalent expression for 12+1 A pizzeria owner wants to know which pizza topping is least liked by her customers so she can take it off the menu. She used four different methods to find this information.Method 1: The owner asked every third customer to rate all the pizza toppings in order of preference.Method 2: The owner gave all customers a toll-free telephone number and asked them to phone in their topping preferences.Method 3: The owner asked the preferences of every other teenager who entered the pizzeria.Method 4: The owner reviewed the pizzerias complaint cards and assessed all complaints related to pizza toppings.Which method is most likely to give a valid generalization? Factoring Perfect Square Trinomials a^2- 2ab + 4b A spinner has the numbers 11-20 on it. What is the probability that it will land on a multiple of 3? 14^-3 x 14^12positive exponents Piedmont Company purchased merchandise on account from a supplier for $45000, terms 1/10, n/30. Piedmont Company returned $7000 of the merchandise and received full credit.If Piedmont Company pays the invoice within the discount period, what is the amount of cash required for the payment A cone has a base diameter of 20 centimeters. Its height is 30 centimeters. Calculate the volume in cubic centimeters to the nearest tenth What is the approximate surface temperature of the 'White Dwarfs'?.2,500-5,000 K5,000-10,00010,000-15,000 K15,000 K-35,000 K What is the GCF of the terms of 3x-9x-12x? Find the SUM of the perfect square roots that fall between the square root of 26 and the square root of 70. What is the area of this quadrilateral?4 ft3 ft10 ft10 ft314 ft how did the rise in deaths impact the american supporters for the vietnam war what is 1000x45 how do i get the anwser Which of the three religions was founded most recently IslamChristianityJudaism?? My Samsung charger broke so I went to Five Below to get a new one. They were on sale for $15,35 (not so five below). Therewas a 6% tax too. How much did the new charger cost? ( I dont need the explanation, only the right answer)