what are the three limitations of an ELISA

Answers

Answer 1

Answer:

Enzyme-linked Immunosorbent Assay, also know as ELISA are used to determine if an antibody is present in a patient's blood sample. They are found in bodily fluids.

Explanation:

The three important limitations are:

1) A positive result confirming a presence of an antibody but it not necessarily making the patient sick

2) A false negative where the amount of antibodies is too low to be measured

3) Another positive result may occur if an unrelated antibody reacts with the antigen non specifically.

Answer 2

The most common limitations of ELISA is based on the antibodies that are being detected..

Enzyme-linked immunosorbent assay (ELISA) is a advanced laboratory test procedure which is used to detect proteins known as antibodies. These antibodies are proteins that are generated in immune system response to antigens of invading microorganisms.

There are some limitations or restrictions seems while performing ELISA test includes:

A false positive result may occur if an unrelated antibody reacts with the antigen non-specifically. When there are false positives in persons that has recovered from a disease. This is because the body can continue to produce antibodies even when the person had recovered.When there is false negatives. This occurs when patients may be poor producers of an antibody or may have some interfering substances in their blood.

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Related Questions

Name 3 considerations you need to keep in mind when deciding an outdoor location for the chicken coop?

Answers

Answer:

1. is the place big enough

2.will they be safe from preaders

3. how well will they respond to a new envorment

Does the fact that a theory changes over time make it less valid science? Why or why not?
Essay

Answers

A theory does not change into a scientific law with the accumulation of new or better evidence. A theory will always remain a theory; a law will always remain a law. Both theories and laws could potentially be falsified by countervailing evidence.

No, the fact that a scientific theory changes over time does not necessarily make it less valid.

What is a scientific theory?

The evolution of scientific theories is an essential part of the scientific process. Scientific theories are based on empirical evidence and are subject to constant testing, refinement, and revision as new evidence becomes available.

The fact that a scientific theory changes over time reflects the scientific community's ongoing efforts to improve our understanding of the natural world based on new observations and data. As more evidence is gathered and analyzed, theories may be modified or even replaced with new explanations that better fit the available evidence.

Therefore, the evolution of scientific theories over time is a sign of the strength and validity of the scientific method, rather than a weakness. It shows that scientists are willing to revise their ideas and hypotheses based on new evidence, and that the scientific community is committed to constantly improving our understanding of the natural world.

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TRUE/FALSE: Incentive theory supports the claim that people are motivated to engage in certain behaviors based on what they think the outcome will be.

Answers

Answer:

False

Hope this helps!

Answer:

Incentive theory supports the claim that people are motivated to engage in certain behaviors based on what they think the outcome will be. TRUE.

(1 ) According to the surgeon general, women should not drink alcoholic beverages during pregnancy because of the risk of birth defects .
(2) Consumption of alcoholic beverages impairs your ability to drive a car or operate machinery and may cause health problems.

Why do you think this warning was added to alcoholic beverages ?

Answers

Public should be informed and reminded about the dangers and health hazards of alcohol consumption.

Skipping a lot of meals can lead to which of the following?

A. A consistent heart rate

B. A steady level of energy

C. The body burning muscle

D. An increase in blood pressure

Answers

Answer:

D an increase in blood pressure

Answer:

I believe It's C, lmk if I'm wrong

table setting worksheet​

Answers

Answer:

this is the answer to your question

Which of the following are considered minor injuries? (Select all that apply.)
A. Broken leg

B. Choking

C. Elbow scrape

D. Slight burn

E. Splinter

Answers

Answer: C. Elbow scrape and E. Slinter

Explanation:

elbow scrape splinter and slight burn. all of these shouldn’t have issues healing. please give me brainliest i only need one more to get my next rank


HELP ME ASAP I NEED HELP WITH THIS QUESTION SOMEBODY ANSWER IT FOR ME PLEASE!!!!!

Suzi goal is to run a 10 mile race in 6 weeks. She hasryt run at all in the last
6 months. Which would be the best way for her to prepare?

A. Run 5 miles 3 times a week for then next 6 weeks

B. Run 1 mile every day for the next 6 weeks

C. Start by running 1 mile and then gradually increase the distance until she can run 10 miles

D. Run 10 miles every day for the next 6 weeks

Answers

Answer:

C

Explanation:

She will increase her stamina by starting with 1 mile and working her way up to 10. All of the other answers either under-prepare her or over-exert her, leading to potential injury.

What tone do these words from the story support? complete the chart. (words from the chart) powerful wave, strength inside) . CONFIDENT ,ANGRY ,TRUSTWORTHY OR SELFISH (i ready)

Answers

The answer is Confident

Answer:

The Answer is Confident. Chart below

Explanation:

When choosing between continuing an argument and walking away, which situation supports walking away as the better option?

A trusted friend is mediating.
There is pressure to act quickly.
The argument is likely to be resolved.
Both individuals are speaking respectfully.

Answers

Answer:

B.) There is pressure to act quickly

Explanation:

Option A is incorrect

there is no conflict.

Option B is correct

if a situation is escalated to the point where one feels pressured to act quickly, leaving the situation altogether would be the best option, instead of continuing it.

Option C is incorrect

there is communication and understanding between the parties that is leading towards a solution. There would be no reason to walk away.

Option D is incorrect

if an altercation is handled in a respectable manner, walking away wouldn't be necessary to diffuse things.

If both individuals are speaking respectfully, then it would be better to continue the argument and try to reach a resolution. Therefor, option (D) is correct.

Why having a conversation important?

Walking away from a respectful conversation can make the other person feel unheard or disrespected, and it may prevent the two individuals from reaching a mutually beneficial agreement. Walking away may be a better option if there is pressure to act quickly and there is no time to discuss the issue thoroughly, or if the argument is likely to escalate into a more heated or aggressive confrontation.

In such situations, it may be more productive to take a break, cool down, and revisit the conversation at a later time when emotions are not running as high. If a trusted friend is mediating, it may be beneficial to continue the conversation with their guidance and support.

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HELP ASAP PLSSSZZZZZ

Answers

testosterone is C.
Sperm is B.
Cervix is F.
Semen is H.
Testes is I.
Ovaries is E.
Zygote is J.
Uterus is G.
Vagina is D.
Penis is A.

____ is the ability to put yourself in someone else's place and imagine how they are feeling.
Love
Empathy
Apathy
Respect

Answers

Empathy that’s the answer yeah yeah u know how it is yeah man
Empathy because we ain't selfish and take care of other people

your overall body type is determined by

heredity
age
gender
activity level
no links please ​

Answers

answer:

heredity.

why?

if your parents are tall you are most likely talk and this is called heredity

Answer:

A.) Heredity

Explanation:

I'm pretty sure the body type is heredity because you inherit that from genes you get from your relatives such as your parents and so on.

Buckwheat is related to

com.

wheat.

quinoa.

rhubarb.

Answers

Rhubarb is what buck wheat is related to

Buckwheat is related to the rhubarb. thus, option D is correct.

What family does buckwheat belong to?

The polygonaceae are a family of flowering plants known informally as the knotweed family or smartweed.

Buckwheat is a pyramid-shaped kernels are similar to grains from both a culinary and nutritional perspective.

It is herbaceous plant of the family Polygonaceae and its edible seeds. Buckwheat is a staple pseudograin crop in some parts of eastern Europe, where the hulled kernels, or groats, are prepared as kasha, cooked and served much like rice. so, option D is correct.

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All of the following can be substituted for fat in baking EXCEPT

tofu.

yogurt.

shortening.

applesauce.

Answers

Tofu can not be substituted for fat in baking

What is the correct definition of an enabler?

A. a gathering where family and friends get a problem drinker to seek help

B. a person who creates an atmosphere for an alcoholic to continue the behavior

C. the process of learning to live without alcohol

D. the physical process of freeing the body of an addictive substance

Answers

Answer:

B

Explanation:

Enabling someone doesn't give them consequences that would discourage negative behavior such as an alcoholic continuing to drink.

Hi! I'm a 17 year old soon to be 18 in a few months. I need to find a place to live (I live in Florida) as I can't live with my parents anymore for inevitable reasons. I don't have a job but I will be getting one. I go to college and I receive full financial aid. I don't have my license yet or a car either or money. But I need to move. Is there any program or help in Lee County that can help me find housing maybe an apartment cheaply or with some time of financial aid. Even thought I will get a job once I turn 18 I won't make enough to pay rent, but a car, pay insurance, pay my phone, food etc... Please all the help you guys can tell me of would be amazing!

Answers

Answer: this is all i could find srry if it dont help enough nd i wish you all the best nd i hope you find yourself housing

Explanation: The Neighborhood Stabilization Program helps income qualifying homebuyers purchase homes in specific areas of Lee County. The Repair and Rehabilitation Programs help income-qualifying homeowners with home repairs if they live in unincorporated Lee County (the areas that are not within any city limits).

If you live on campus and receive financial aid, that should include housing, no?

The _____________ also secretes a fluid that helps the sperm move; it is located just past the ejaculatory duct and surrounds the urethra.

a. seminiferous tubules

b. epididymis

c. vas deferens

d. prostate gland

e. seminal vesicles

Answers

e. seminal vesicles random letters usbaibajan

The
are the primary organs of the lower respiratory system.

branch(es) into the lungs, allowing for the maximum amount of space for oxygen to be moved into the blood.
The
is the fluid-filled space that surrounds the lungs.

Answers

Answer:

i believe so

Explanation:

Answer: The LUNGS are the primary organs of the lower respiratory system.

BRONCHIOLES branch into the lungs, allowing for the maximum amount of space for oxygen to be moved into the blood.

The PLEURAL CAVITY is the fluid-filled space that surrounds the lungs.

Explanation:

It’s right trust me

The
controls our voluntary actions?
Autonomic nervous system
Limbic system
Parasympathetic nervous system
Somatic nervous system

Answers

Answer:

All the body's voluntary actions are controlled by the motor cortex in the frontal lobe of the cerebrum

3 ways mental health can be connected to substance use problems?

Answers

Answer:

Having an overwhelming amount of stress or anxiety and thinking drinking will end the pain, an overdose of a prescribed drugs or having thoughts of self harm and drinking to end one's life.

Explanation:

How do you make popcorn?

Answers

Answer:

microwave it

Explanation:

If an electron vibrates back and forth in an clean wire with a frequency of 60.0 Hz, how many cycles make in 1.0 h?

a. 8.1 x 10^5

b. 6.0 x 10^2

c. 3.7 x 10^3

d.2.2 x 10^5

e. 4.6 x 10^4

give atleas five components to be measured computer system servicing​

Answers

Answer:

motherboard

central processing unit (CPU)

RAM

ROM

graphics processing unit (GPU)

Identify the sentence that uses noun/pronoun agreement correctly. (1 point) Group of answer choices Our dogs Bear and Bryant lost all ability to think when they sniffed a rabbit on the hunt this morning. Our dogs Bear and Bryant lost all ability to think when he sniffed a rabbit on the hunt this morning. Our dogs Bear and Bryant lost all ability to think when we sniffed a rabbit on the hunt this morning. Our dogs Bear and Bryant lost all ability to think when she sniffed a rabbit on the hunt this morning.

Answers

Our dogs Bear and Bryant lost all ability to think when they sniffed a rabbit on the hunt this morning.

Can anyone course hero this please i’ll give brainiest points please!!!! ASAP!!!!

Answers

Answer: Cystic Fibrosis: usually from both parents,  usually affect both,  signs and symptoms- Very salty-tasting skin.

Persistent coughing, at times with phlegm.

Frequent lung infections including pneumonia or bronchitis.

Wheezing or shortness of breath.

Poor growth or weight gain in spite of a good appetite.

Frequent greasy, bulky stools or difficulty with bowel movements.

Male infertility.  frequency- Frequency. Cystic fibrosis is a common genetic disease within the white population in the United States. The disease occurs in 1 in 2,500 to 3,500 white newborns. Cystic fibrosis is less common in other ethnic groups, affecting about 1 in 17,000 African Americans and 1 in 31,000 Asian Americans. treated- antibiotics to prevent and treat chest infections. medicines to make the mucus in the lungs thinner and easier to cough up. medicines to widen the airways and reduce inflammation. special techniques and devices to help clear mucus from the lungs. Average life- Today, the average life span for people with CF who live to adulthood is about 44 years. Death is most often caused by lung complications. birth- Prenatal diagnostic tests to detect CF and other disorders include amniocentesis and chorionic villus sampling (CVS). Amniocentesis usually is done between 15 and 20 weeks of pregnancy, but it also can be done up until you give birth.  Your doctor may perform a prenatal screening to test for CF if both parents know they carry the gene. This is performed while you are pregnant. It checks for any health problems with your baby before he or she is born. The screening also can detect other genetic disorders

Explanation:

What are the two main types of interventions that are used to treat diseases and disorders?
surgical treatments, pharmacological treatments
O physical therapy, mind and body medicine
O body-based practices, psychotherapy
O conventional medicine, complimentary and alternative medicine

Answers

Answer:

preventive interventions and therapeutic interventions

Explanation:

Which materials are part of a person’s body composition? Select three options.

fat
supplements
water
protein
medications

Answers

Answer:

Fat, water and protein

Explanation: They are the core components or things that we need in our body.

Answer:

protein, Fat, and water

:3 can i have a brainliest, plz

i need help...........

Answers

Abstinence

This is the most effective method. It is the only way to ensure that pregnancy won't happen.

Diaphragm

This contraceptive is a flexible plastic cup that is inserted before sexual activity.

Sterilization

This method involves a vasectomy in males and a tubal ligation in females.

Implant

This contraceptive is inserted under the skin of a woman's upper, inner arm.

which food would help other food move through the digestive system

Answers

Answer:Vegetables are rich in fiber, which is an important nutrient for digestion. Fiber stimulates the bowels to move stool out of the body. The skins of vegetables are often rich in fiber, and it is best to consume them whole. Some vegetables with skin rich in fiber include potatoes, beans, and legumes.

Answer:

Vegetables are rich in fiber, which is an important nutrient for digestion.

Explanation:

Fiber stimulates the bowels to move stool out of the body.

How do viruses reproduce?

by taking over the machinery inside a host cell to make more viruses

by sharing DNA with other, nearby viruses

through binary fission

through sporulation

Answers

Answer:

I believe it's the first one. Viruses need to infect cells in order to mulitiply.

Explanation:

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