What are three methods for detecting radioactively labeled DNA in an experiment?

Answers

Answer 1

Answer:

Limitations. detect low abundance DNA binding proteins from lysates. test binding site mutations using many probe configurations with the same lysate. test binding affinity through DNA probe mutational analysis. non-radioactive EMSA possible using biotinylated or fluorescently labeled DNA probes.

Explanation:

Answer 2

Answer:

Proteins interact with DNA through electrostatic interactions (salt bridges), dipolar interactions (hydrogen bonding, H-bonds), entropic effects (hydrophobic interactions) and dispersion forces (base stacking). These forces contribute in varying degrees to proteins binding in a sequence-specific or non–sequence-specific manner.

Explanation:


Related Questions

Which factor would have the greatest influence on the southern migration of animals living in the northern hemisphere

Answers

Answer:

Animal migration is the relatively long-distance movement of individual animals, usually on a seasonal basis. It is the most common form of migration in ecology. It is found in all major animal groups, including birds, mammals, fish, reptiles, Many of these migrations are north-south, with species feeding and breeding in.

Photosynthesis is related to cellular respiration and why?

Answers

Answer:

Explanation:

Photosynthesis makes the glucose that is used in cellular respiration to make ATP.  While photosynthesis requires carbon dioxide and releases oxygen, cellular respiration requires oxygen and releases carbon dioxide. It is the released oxygen that is used by us and most other organisms for cellular respiration. :)

If I were to make hot cocoa which consists of chocolate syrup and milk, identify the solution
a. chocolate syrup
b. milk
c. none of the above
d. hot cocoa

Answers

D. Hot cocoa is the solution=answer
i believe the answer is d

What is the correct answer?

Answers

Answer:

The object is at rest.

Thank you and please rate me as brainliest as it will help me to level up

I want to know know know know

what is love ☕

Answers

Answer:

Love = Silence

Explanation:

Because sometime you can't tell someone how you feel so you have to keep your love silenced.

How many different kinds of atoms does an element contain?

A) 2
B) 1
C) 8
D) 4

Answers

Answer:

an element contain 2 atoms

Answer: Your just a kid who doesn't know anything

How are organisms able to generate ATP without oxygen?

Answers

Answer:

Without oxygen, organisms can split glucose into just two molecules of pyruvate. This releases only enough energy to make two ATP molecules. ... This releases enough energy to produce up to 38 ATP molecules. Thus, aerobic respiration releases much more energy than anaerobic respiration.

True or False?

Chemistry is about patterns. The Periodic Table contains, demonstrates, and reveals those patterns.

Answers

Answer:

Are metals and non metals

The following are all examples of changing motion EXCEPT

a
A golf ball stops after rolling up a hill.
b
A race car travels around an oval track at a constant speed of 180 miles per hour.
c
A bowling ball travels in straight line down a bowling alley at a constant 3 meters per second.
d
A ball rolls against a wall and bounces away at 3 meters per second.

Answers

Answer:

It is B

Explanation:

I had this on my test last week

In the following diagram of boiling water, what is causing the rising and sinking of the water?

A electromagnetic radiation from the atmosphere
B the insulation of water due to its high heat capacity
C convection from the movement of liquids and gases
D conduction from the fire underneath the pot

Answers

Answer:

B.

Explanation:

the insulation of water due to its high heat capacity

Before deciding on a medium to use, what should the presenter do?(1 point)

compare the strengths and limitations of each medium

decide on the number of still or moving images they need

know the age and size of their audience

chose the form they feel most comfortable with

Answers

Answer:

♡ madeline here! ♡

- -it's c ʕ •ᴥ•ʔ

hope I could help! have a great day

✧・゚: *✧・゚:・゚✧*:・゚✧・゚: *✧・゚:・゚✧*:・゚✧

.

Explanation:

Answer:

A) Comparing the strengths and limitations of each medium

Explanation:

That's what is said on the lesson and this is 100% right!

What eats a parrotfish? Does a great white shark count‍♂️

Answers

Answer:

Shark eats a parrotfish. No but help to maintain the populations of the next-biggest predators in the food chain.

Explanation:

The nitrogen, oxygen, and other gases surrounding Earth
A.are pulled toward Earth by gravity
B.are found mainly near the outer edge of the atmosphere
C.exert pressure only at sea level
D. exert pressure that works against atmospheric pressure

Answers

Answer:

option A is correct

Explanation:

Which statement best explains the shape of these layers of rock?
O A. Stress caused by forces that pull on both sides of an area of the
crust made the rock melt.
O B. Stress caused by tectonic plates moving at a transform boundary
made the rock break.
O C. Stress caused by a collision between two tectonic plates made the
rock bend
O D. Stress caused by the weight of the upper layer of rock made the
rock tilt

Answers

Answer:

C. Stress caused by a collision.........made the rock bend.

Answer:

C. Stress caused by a collision between two tectonic plates made the rock bend

Explanation:

The human genome project was produced an area of science known as bioinformatics. how is this helpful in sequencing DNA?

Answers

Bioinformatics is a subdiscipline of biology/computer science concerned with the acquisition, storage, analysis, and dissemination of biological data. This helps organize and determine nucleotides of a given DNA molecule from known data about biological data through computer sustems.

** The Human Genome Project increased speed of DNA sequencing and lowered the cost. Basically made it more efficent.

Check out Bioinformatics lectures and websites! You will gain a better understanding of how this is helpful for sequencing DNA.

PLEASE HELP ME!!!!!!
What happens to the muscles when the weight is lowered?

Answers

Answer:

C is the answer to the question

7(c)(i). The diagram 7.2 shows the materials used by Claudia for preparing
tea. Claudia finds that the colour of the tea is light. Why? *​

Answers

Answer: Because she used cold water

Explanation:

Tea leaves contain different flavors that dissolve in water to give it its distinct taste as well as its color. If water is hot, the flavors will dissolve quickly due to the fast movement of the heated water molecules.

If the water is cold however, as is the case in the above scenario, the flavors will take longer to dissolve in the water which means that the tea's color will not change drastically leading to a light tea.

is centrioles a plant or animal cell?​

Answers

Answer:Animal cells but some plant cells also have it.

Explanation:

why is phosphorous an important cycle on the earth and how does it have little correlation to human life

+30pts and brainliest

Answers

Answer:

aaaaaaaaaaaaaaaaaaaaaaaaa6rytr

16 An organism with a cell wall would most likely be unable to take in materials through
A) osmosis
B) facilitated diffusion
C) active transport
D) endocytosis​

Answers

Answer:

endocytocis

Explanation:

I am pretty sure that is B but it can also be D

Messenger RNA (mRNA) contains the instructions for —
A. building mitochondria
B. protein assembly
C. DNA replication
D. RNA synthesis

Answers

Answer B, explanation: The mRNA directs the synthesis of proteins, which occurs in the cytoplasm. mRNA formed in the nucleus is transported out of the nucleus and into the cytoplasm where it attaches to the ribosomes. Proteins are assembled on the ribosomes using the mRNA nucleotide sequence as a guide.

please help me, i dont like this :(

Answers

Answer:

This is false.

Explanation:

Plants are eukaryotes, meaning they contain a nucleus in their cells. Prokaryotes, such as bacteria, do not contain a nucleus, and their genetic material, better known as DNA, floats about freely in their cytoplasm.

The answer to this is false

16
Select the correct answer.
Which type of evidence is often reviewed in microscopy?
OA physical evidence
OB.
trace evidence
OC. personal experience
OD
testimonial evidence
OE.
circumstantial evidence

Answers

Answer:

B. Trace Evidence

Explanation:

Trace evidence is like fiber or hair or gunshot residue to it would be B. Trace evidence

The physical evidence and trace evidence are both reviewed under microscopy. Therefore, option A and option B are correct statements.

What is microscopy?

Microscopy is a technique to review the smaller size object at high magnification. Forensic science uses the physical or trace evidence found at the crime scene to review under microscopy.

Physical evidence refers to the tangible objects found at the crime scene, such as murder weapons or tool marks. On the other hand, trace evidence refers to the small or even microscopic pieces of evidence left at the crime scene, such as hair, or DNA.

Using the microscopy technique, the forensic team can determine the source of the murder weapon used at the crime scene or even link the suspect to the death of the victim. However, other types of evidence that do not come under microscopy, such as testimonial evidence or circumstantial evidence are also used to build a strong case against the suspect.

Therefore, the physical evidence and the trace evidence both are reviewed under microscopy.

Learn more about microscopy here:

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what is the average for a human piens A 2.inc B 4inc C 10 inc D 1 feet

Answers

Answer:

I'm gonna have to go with B 4 inches

Explanation:

it is B cause I'm below average and no one is longer than 10 inches

Why is the production possibility curve drawn as a bowed-out curve?

Answers

Answer:

he downward slope of the production possibilities curve is an implication of scarcity. The bowed-out shape of the production possibilities curve results from allocating resources based on comparative advantage.

Explanation:

How do you know that energy and matter are conserved during the process of cellular respiration?

Answers

Answer:

Energy and matter are conserved in cellular respiration because it has identifiable inputs and outputs. These can be modeled with chemical equations.

Food undergoes chemical reactions in organisms to generate new molecules for growth or energy. Therefore, energy and atoms are conserved during cellular respiration.

What is conservation of energy and matter in cellular respiration?

The law of conservation of energy states that energy cannot be created or destroyed but can be changed from one form to another. The law of conservation of matter states that the total mass within a closed system that does not allow matter or energy to escape should remain constant. However, the law of conservation of energy states that energy can change from one form to another.

The process of cellular respiration is an example of a chemical reaction, which involves the transformation of one type of matter into another. A chemical equation describes this change in molecular structure. This is the cellular respiration chemical equation:

[tex]C_{6} H_{12} O_{6} + 6 O_{2} = > 6CO_{2} + 6H{2}0 + energy[/tex]

(Glucose + Oxygen yields Carbon Dioxide + Water + Energy)

Therefore, the amount of carbon dioxide atoms released by respiration is the same as the amount of  atoms in the oxygen and carbon that were changed.

Learn more about cellular respiration, here:

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Which of the following is a pluton?
A. Pyroclast
O
B. D*ke (it's a type of rock not the slur)
O
C. Lahar
O
D. Lava flow​

Answers

Answer:

The correct answer is d*ke

Explanation:

I tried the other answer and got it wrong, this was the right one on the test.

When the accused enters into plea bargaining, the judge and prosecutor do not have to hold a trial. True or False

Answers

Answer:

True

Explanation:

Answer:

False

Explanation:

Put true on edge got it wrong its false

How does the muscular system help humans maintain posture?
1.Muscles continually align themselves vertically to help maintain posture.
2.Muscles continually push bones to be straight.
3.Muscles constantly contract to help fight gravity.
4.Muscles constantly contract to help work with gravity.

Answers

Answer:

a

Explanation:

Muscles continually align themselves vertically to help maintain posture.

What is Muscular system?

Muscle fibers are specialized cells that make up the muscular system. Their ability to contract is its main characteristic. Movement is caused by muscles that are connected to blood vessels, internal organs, or bones.

Muscle contraction is the primary cause of almost all bodily movement. Cilia, the flagellum on sperm cells, and certain white blood cell amoeboid movement are examples of exceptions to this rule.

Walking and running are two examples of visible movements that are produced by the coordinated action of joints, bones, and skeletal muscles. Skeletal muscles can also make softer motions that influence different facial expressions, eye movements, and breathing.

Therefore, Muscles continually align themselves vertically to help maintain posture.

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what element has 7 protons and 5 neutrons?

Answers

Nitrogen because it has an atomic number of 7 and also 7 protons
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