What is chromotography and how does it work​

Answers

Answer 1

Answer:

chromotography: It is a process of quick and effective separation and purification of components of a combination. It bases its migration through a stationary phase, under the influence of a moving period, of the various components of the mixture.

Explanation:

How does chromatography work?

The same basic idea applies to all types of chromatography. The mobile phase, as its names suggest, is "mobile" and passes through a stationary phase. The different components of the mixture are divided between stationary and mobile phases, while the moving mobile phase passes through the stationary. This procedure allows components to be separated into a blend because the various components of the blend have various interactions with the mobile and stationary phases.

In reality, this means that in combination components that 'stick tighter' to the fixed phase remains in the fixed phase for a longer period. This means that the components in the mixture are separated, which are not 'strongly held' in the stationary phase. This is the basis of a separating technique for chromatography.

For instance, cotton wool or a glass fried sinter are placed at the base of a glass column in a simple liquid column chromatography system. The column then has a silica package. The column is packed in many ways, e.g. dry packaging or slurry. The crucial factor is that silica is well-packed without air bubbles or crashes, which prevents the movement of mobile phases. The mobile phase, often referred to as the eluent, is then added to reach the top of the silica, once the sílice is well packed. Following this, the mixture that is going to be separated is gently added to the top of the saturated silica. A layer of sand is then placed onto the mixture to ensure it is not disturbed by the addition of more eluent. The eluent (the mobile phase), which can be a solvent or mixture of solvents, is then continually introduced to the system until the mixture has been separated. It is crucial to make sure the eluent level never drops below the top of the silica to prevent air bubbles from entering the stationary phase and inhibiting mobile phase flow.

The components of the blend that "stands tight" in the steady phase (in this example silica) will stay longer in the column as the eluent is flushed down the column. Instead, components which do not 'close' to the stationary phase are faster to elute from the column and gather as fractions. It goes on until all the components have eluted individually from the column. The consequence is a separate mix.

Chromatography can work as a separation technique, as the stationary and mobile phasing attractions of distinct components in a mixture. Due to the varied qualities of the components in the combination, the different attractions are.

Silica, a stand-alone common phase, has Si-O-H connections on the surface. Si-o bonds and hydroxyl groups represent an extremely polar silica gel surface (stationary phase) able to form hydrogen bonds, and can also take part in the interactions of van der Waals, dipole–dipole and dipole–induced dipole.

As the complex mixture travels through the column, how quickly the various components of the elute from the column depend on two characteristics: 1) the degree of stabilisation of each chemical. The interactions between the various components and the silica gel depend on that. 2) How soluble in the mobile phase are the constituent components (the eluent). This relies on how the components interact with the solvent system.

While numerous components in a combination may be extremely similar and even possible to bind hydrogen to silica during the steady-stage stage, hydrogen binding on a silica is highly rare and will interact exactly the same way with the mobile phase. Co-elution (with multiple elements elucidating from the column concurrently) may occur in complex chromatographic separations. Different solvent systems and pH altering tests can even separate some of the most complicated blends into distinct components with the simple chromatography of the liquid column, by means of extensive and meticulous modification in the mobile phase.


Related Questions

Hello, can someone please help me with this!

Answers

Explanation:

Reconstruction was the period in the united states during which the Civil war took place and the laws were made to provide equity to the slaves ending slavery.

The problem arises due to the reunion of the 11 states of the South to the Union which seceded earlier.  Slavery was more practised in the Southern states compared to Northern states.

The Republican party did corruption from 1869 to 1877 which allowed due to which they lost their election of 1877.

In 1877, a special electoral commission was established in favour of Hayes and Democrats took benefits of this condition.

When Hayes was selected, he withdrew the federal troops from the South which allowed the Democrats to gain their seats in South and ended Reconstruction.

The nitrogen bases down the center are held together by

Answers

Answer:

Hydrogen Bonds

Explanation:

The nitrogen bases are held together in the center of the molecule by Hydrogen Bonds. The type of sugar found in DNA is Deoxyribose.

Answer:

hydrogen bonds

PLEASE HELP WITH #2 complete sentences please I will mark brainliest

Answers

It will take Kira 12.5 seconds to run the 100 meter hurdles.

please help me answer!!

Answers

Spots of germs and bacteria will be on the wall it’s called a uv light and it’s used for crime scenes to spot blood stains

students have a mixture of small iron filings and grains of sand that are about the same size. how can the students most easily separate the parts of this mixture?
A. By dissolving the sand in water
B. By picking out the grains of sand by hand
C. By using a magnet to attract the iron filings
D. By using a screen to filter out the iron filings

Answers

The answer would be C because iron is magnetic

Answer: c

Explanation:

Prokaryotic cells do not contain many of the organelles that eukaryotic cells have. Prokaryotic cells do have a primitive form of

ribosomes
chloroplasts
mitochondria
Golgi apparati

Answers

Answer:

ribosomes

Explanation:

the only common organelle between the eukaryotic and prokaryotic cell

the primitive prokaryotic ribosomes are 70S while the eukaryotic ribosomes are 80s

In snapdragon the gene that affects flower color has two alleles: red (R) and white (W). In heterozygous individuals, the phenotype is pink. If two pink flowers were crossed what would be the probability of a pink offspring?

A. 25%

B. 50%

C. 75%

D. 100%

Answers

Answer:

The correct answer is - 50%

Explanation:

In the heterozygous condition, there is always one allele of dominant and one allele of recessive present together and during gamete formation, they assort independently. The probability of the pink flower that is RW can be found by punnet square:

      R     W

R    RR   RW

W   RW    WW

here, out of four two cases is of heterozygous or pink flower so the ration would be 2/4 or 1/2 or 50%

what is the shape of dna in a prokaryotic cell

Answers

Prokaryotic chromosomes are found in the nucleoid of prokaryotic cells, and they are circular in shape

Someone answer plssss​

Answers

Answer:

parent cell                          diploid cells

Explanation:

Answer:

1. mother cell

2. daughter cells

Explanation:

I hope that helps!

What cells combine to make the first eukaryotic cell?

Answers

Explanation:

Eu" means "true," and eukaryotes have DNA arranged in chromosomes and encased in a nucleus. Fossil evidence indicates that prokaryotic cells first existed on the earth, prior to the arrival of the eukaryotes.

eukaryotic cells evolved from a symbiotic association of prokaryotes—endosymbiosis—is particularly well supported by studies of mitochondria and chloroplasts, which are thought to have evolved from bacteria living in large cells.

_____ is a molecule of oxygen with


_______oxygen atoms instead of 2.

Answers

Answer:

Ozone, 3.

Explanation:

Ozone is a molecule of oxygen with  3 oxygen atoms instead of 2 that is present in the atmosphere of the earth. It is a very important gas because this gas filter the solar radiation  from ultra violet radiation which causes skin cancer disease. Ozone is present in the stratosphere layer of atmosphere that is present between 15 to 30 kilometer high from the earth surface.

Which of the following best describes the role of ATP in cell repair?
A)
ATP acts like a patch to repair damaged areas.
ATP creates new cells to replace the damaged ones.
C)
ATP directs the enzymes responsible for repairing cells.
D)
ATP supplies energy needed for repairs.

Answers

Answer:

D.

Explanation:

ATP is responsible for supplying needed energy for the repairs to occur.

They are a reliable energy source.

Hope this is correct, have a great day.

What agent of deposition is this? Gravity,wind, waves, running water or glaciers.

Answers

Answer:

Sediment

Explanation

Maybe it is Glaciers ?

Name 3 sources of CO2.

Answers

Answer:

1. Natural Source

2. Human Source

3. Natural Gas

I don't know if the third one is correct...

just do the first one and if you don’t know it don’t answer please this is due today

Answers

The first one is evaporation, condensation, precipitation, and collection. (Hope it helped)

How many atoms of carbon, hydrogen, and oxygen are there in the reactants of photosynthesis?

Answers

Answer:

The reactants have one carbon atom, four hydrogen atoms, and four oxygen atoms. IN HYDROGEN ATOMS  It takes six atoms of carbon (C) and twelve atoms of hydrogen (H) to make one molecule of glucose (C6H12O6). These atoms come from carbon dioxide (CO2) and water (H2O). IN OXGYEN It takes six atoms of carbon (C), six atoms of oxygen (O) and twelve atoms of hydrogen (H) to make one molecule of glucose (C6H12O6).

In photosynthesis, the reactants have one carbon atom, four hydrogen atoms, and four oxygen atoms.

What do you mean by photosynthesis?

Photosynthesis is a process used by plants and other organisms to convert light energy into chemical energy that, through cellular respiration, can later be released to fuel the organism's activities.

Photosynthesis is the process in which light energy is converted to chemical energy in the form of sugars. In a process driven by light energy, glucose molecules (or other sugars) are constructed from water and carbon dioxide, and oxygen is released as a byproduct.

Photosynthesis requires sunlight, carbon dioxide, and water as starting reactants. After the process is complete, photosynthesis releases oxygen and produces carbohydrate molecules, most commonly glucose. These sugar molecules contain the energy that living things need to survive.

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Several factors determine the distribution of biomes on the planet. Which factor is considered most important?

A. weather
B. temperature
C. altitude
D. precipitation

Answers

Answer:

B

Explanation: i hope this is the answer

From the equator to the poles, the temperature of the Earth's surface normally drops. A biome's moisture content is influenced by both precipitation and evaporation, hence option B is correct.

What are biomes?

Climate is the most important abiotic factor affecting the distribution of terrestrial biomes.

Temperature and precipitation are the primary determinants of plant growth. The plant will be discovered at that temperature and precipitation will be determined by the biome's temperature.

A biome's climate is mostly determined by the forms of life that exist there. Latitude, geographic characteristics, and atmospheric processes that disperse heat and moisture are only a few of the variables that affect climate. Scientists disagree on the number of distinct biomes that exist.

Therefore, temperature determines the distribution of biomes on the planet.

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How many hydroxl groups does psilocybin have?

Answers

Answer:

One

Explanation:

How is fitness related to genes?
a. Animals with higher fitness have a better chance of
passing their genes on to the next generation.
b Animals with higher fitness make better and more
useful genes.
c Animals with lower fitness have a better chance of
passing their genes on to the next generation.
Need a Hint?
d. Animals with higher fitness use their genes more
efficiently.

Answers

Answer:

b

Explanation:

Genes passing to the next and third generation

Explain an example of how artificial selection can help solve human problems.

Answers

Artificial selection appeals to humans since it is faster than natural selection and allows humans to mold organisms to their needs.

Answer:

Artificial selection can help solve human problems since humans can choose the desired traits of an organism to coordinate with their needs.

Explanation:

Since artificial selection is predictable, it is easier for humans to "customize" the traits of an organism to get a desired trait.

Which organism is defined as something that lives deep in the ocean in areas with very high temperatures and pressure?

Answers

Answer:

shark

Explanation:

Organisms living in the deep ocean must be adapted to survive under extreme pressure, limited light, cold temperatures, and other factors. Organisms living in the deep ocean must survive in a physical environment that is radically different from ocean habitats near the sea surface.

Which is NOT a form of electromagnetic radiation?

A.
radio waves
B.
sound waves
C.
x-rays
D.
gamma waves

Answers

Answer:

Sound waves

Explanation:

Chemical reactions that happen in the __________ inside chloroplasts

Answers

Answer:

chemical bond

Explanation:

In the USA, many people want to cut their
body mass. Suggest why they
Suggest why they are advised to
avoid cheese.

Answers

Answer:

EATING CHEESE, BUTTER AND CREAM CAN HELP PROTECT YOU FROM HEART DISEASE, STUDY FINDS

Explanation:

Fatty foods such as cheese, butter, and cream are often considered culprits of heart disease – but according to a new study, a diet high in saturated fat could in fact bring significant health benefits.

Eating more naturally high-fat foods while limiting the amount of carbohydrates consumed did not cause an increase in harmful cholesterol, researchers at the University of Bergen in Norway found

3. True or false: Science aims to answer TESTABLE claims.

Answers

Answer: True

Testable claims, or verifiable claims, are claims where we can prove them true or false. We do so by gathering/observing data, then afterward we see if the data fits the claim or not. If not, then we adjust the claim or we throw it out entirely.

What are the possible phenotype(s) for their children? *
A. Hazel eyes
B. Brown eyes

Answers

Answer:

hazel eyes are recessive, Brown eyes are dominant. is there a pedigree along with this question?

Explanation:

Please insert an image of a pedigree

hope this helps!

✧◝(⁰▿⁰)◜✧

Where is the active site located on the enzyme?

Answers

Answer:

The active site located in a deep tunnel within the enzyme, or between the interfaces of multimeric enzymes.

PLEASE ANSWER THISSSSSSSD​

Answers

Answer:

D. Carbon dioxide + water

Explanation:

I hope it helps ❤❤

Adenine base pairs with thymine using _________ bonds, and guanine base pairs with cytosine using ________ bonds.

A) two hydrogen, three hydrogen
B) three hydrogen, two hydrogen
C) three phosphoester, two phosphoester
D) two phosphoester, three phosphoester

Answers

Adenine base pairs with thymine using two hydrogen and guanine base pairs with cytosine using three hydrogen bonds.

What is DNA?

Deoxyribonucleic acid (DNA) is a molecule composed of two strands linked by hydrogen bonds.

In DNA Adenine (A) only pairs with Thymine (T), whereas Guanine (G) pairs with Cytosine (C).

In conclusion, adenine base pairs with thymine using two hydrogen and guanine base pairs with cytosine using three hydrogen bonds.

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Answer:

A) two hydrogen, three hydrogen

Explanation:

If two air masses have the same relative humidity of 60 %, which one contains more water vapor?

a. The one with the higher temperature
b. Both would have the same amount
c. The one with higher relative humidity
d. The one with the lower temperature

Answers

b. Both would have the same amount

Option b. Both contain a similar amount.

Given that,

The two masses should have a similar relative humidity of 60%.We need to find out which have more water vapor.

Based on the above information, we can say that since both two masses contain the same humidity i.e. 60% so it means that they have the same amount.

Therefore the option b is correct.

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